| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Clostridioides | AGGTAAAGCGCAAGGAAGCA | TGCCTCATCAAACTCCTCAAC | 60.25 | 58.22 | 170 | 88 |
100.00%
|
61.54%
|
| Primer 42 | Clostridioides | GGTAAAGCGCAAGGAAGCA | TGCCTCATCAAACTCCTCAAC | 58.75 | 58.22 | 169 | 88 |
100.00%
|
61.54%
|
| Primer 43 | Clostridioides | TGCGGAAGGTCGATTTATTGC | ACCAAAGGCATGCTGAACTC | 59.33 | 58.75 | 270 | 88 |
100.00%
|
61.54%
|
| Primer 44 | Clostridioides | AGTAACAGTTGGTGCAACAGT | GCGGTCTTGCTCCTACACA | 58.00 | 59.71 | 290 | 87 |
100.00%
|
61.54%
|
| Primer 45 | Clostridioides | AGTAACAGTTGGTGCAACAGT | CTGCGGTCTTGCTCCTACA | 58.00 | 59.41 | 292 | 87 |
100.00%
|
61.54%
|
| Primer 46 | Clostridioides | AGTAACAGTTGGTGCAACAGT | TGCGGTCTTGCTCCTACA | 58.00 | 58.21 | 291 | 87 |
100.00%
|
61.54%
|
| Primer 47 | Clostridioides | TGGCAGCTCCATCAACTGT | TACCAAGCCCTGCTCCCAT | 59.24 | 60.62 | 189 | 87 |
100.00%
|
61.54%
|
| Primer 48 | Clostridioides | TGTGCGAAACAGTAGCTGAAA | AGGGCATTTTGTTCAGCGTG | 58.44 | 59.69 | 193 | 87 |
100.00%
|
61.54%
|
| Primer 49 | Clostridioides | TGTGCGAAACAGTAGCTGAAA | GGGCATTTTGTTCAGCGTG | 58.44 | 58.19 | 192 | 87 |
100.00%
|
61.54%
|
| Primer 50 | Clostridioides | TGTGCGAAACAGTAGCTGAAA | AAGGGCATTTTGTTCAGCGTG | 58.44 | 60.27 | 194 | 87 |
100.00%
|
61.54%
|