| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Clostridioides | AGAGGCAATTGGGGAAGACC | AGCCCTGTAAGTCTTCCTCC | 59.67 | 58.43 | 261 | 93 |
100.00%
|
69.23%
|
| Primer 22 | Clostridioides | AGCGCAAGGAAGCAGAAGAT | TGCCTCATCAAACTCCTCAAC | 60.04 | 58.22 | 164 | 91 |
100.00%
|
61.54%
|
| Primer 23 | Clostridioides | GGGTGTGATACAGGCTCATGT | AATCCACACTGGTCAGCCC | 59.79 | 59.62 | 182 | 90 |
100.00%
|
61.54%
|
| Primer 24 | Clostridioides | AGGGTGTGATACAGGCTCATG | AATCCACACTGGTCAGCCC | 59.51 | 59.62 | 183 | 90 |
100.00%
|
61.54%
|
| Primer 25 | Clostridioides | ACAGGCTCATGTGGGTTATGT | AATCCACACTGGTCAGCCC | 59.36 | 59.62 | 173 | 90 |
100.00%
|
61.54%
|
| Primer 26 | Clostridioides | TGCCCATACTGATTTGAGGGA | TCTGGTGTACCTGTTCCTGGA | 58.80 | 60.13 | 291 | 90 |
100.00%
|
61.54%
|
| Primer 27 | Clostridioides | TGCCCATACTGATTTGAGGGA | CTGGTGTACCTGTTCCTGGA | 58.80 | 58.65 | 290 | 90 |
100.00%
|
61.54%
|
| Primer 28 | Clostridioides | TGCCCATACTGATTTGAGGGA | TCTGGTGTACCTGTTCCTGG | 58.80 | 58.65 | 291 | 90 |
100.00%
|
61.54%
|
| Primer 29 | Clostridioides | TGCCCATACTGATTTGAGGGA | TTCTGGTGTACCTGTTCCTGG | 58.80 | 59.30 | 292 | 90 |
100.00%
|
61.54%
|
| Primer 30 | Clostridioides | TGCCCATACTGATTTGAGGGA | AGGTTCTGGTGTACCTGTTCC | 58.80 | 59.30 | 295 | 90 |
100.00%
|
61.54%
|