| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Clostridioides | GACTAGAGGTGTGCCAATTGC | ATGCCCTCTGTCCACTAGC | 59.26 | 58.78 | 295 | 96 |
100.00%
|
61.54%
|
| Primer 12 | Clostridioides | ACTAGAGGTGTGCCAATTGC | ATGCCCTCTGTCCACTAGC | 58.17 | 58.78 | 294 | 96 |
100.00%
|
61.54%
|
| Primer 13 | Clostridioides | GCGCAAGGAAGCAGAAGATG | TGCCTCATCAAACTCCTCAAC | 59.90 | 58.22 | 163 | 95 |
100.00%
|
61.54%
|
| Primer 14 | Clostridioides | CCAGGAGATAAAGGCGATGTG | CTGGACAATGTGCATCTGAGC | 58.51 | 59.60 | 169 | 95 |
100.00%
|
69.23%
|
| Primer 15 | Clostridioides | CCAGGAGATAAAGGCGATGTG | TGGACAATGTGCATCTGAGC | 58.51 | 58.54 | 168 | 95 |
100.00%
|
69.23%
|
| Primer 16 | Clostridioides | GTTGCTGTTGATGCCGACC | ACCACCAGAATCTACGGTTCC | 59.79 | 59.45 | 246 | 93 |
100.00%
|
61.54%
|
| Primer 17 | Clostridioides | AGTAACAGTTGGTGCAACAGT | TGCGGTCTTGCTCCTACAC | 58.00 | 59.71 | 291 | 93 |
100.00%
|
61.54%
|
| Primer 18 | Clostridioides | AGTAACAGTTGGTGCAACAGT | GCTTTCTGCGGTCTTGCTC | 58.00 | 59.50 | 297 | 93 |
100.00%
|
61.54%
|
| Primer 19 | Clostridioides | AGTAACAGTTGGTGCAACAGT | GCGGTCTTGCTCCTACACAT | 58.00 | 60.11 | 290 | 93 |
100.00%
|
61.54%
|
| Primer 20 | Clostridioides | GATGGGGAAAGGCATTGGC | AACGTCTCACTGCAAAGGC | 59.17 | 58.68 | 251 | 93 |
100.00%
|
61.54%
|