| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 581 | Clostridioides | ACAGAATTGAGGGGAGCCA | GTTCCTGTAGTTGGCAGTGGT | 58.21 | 60.20 | 272 | 50 |
100.00%
|
69.23%
|
| Primer 582 | Clostridioides | ACCAACTGGATGGAGCCCT | GACCAGTTCCTGCAGCACA | 60.54 | 60.23 | 299 | 50 |
100.00%
|
69.23%
|
| Primer 583 | Clostridioides | ACCAACTGGATGGAGCCCTA | ACCAGTTCCTGCAGCACA | 60.25 | 59.08 | 298 | 50 |
100.00%
|
61.54%
|
| Primer 584 | Clostridioides | AGGGGTGCAAAGTCTGATGTT | TGAGCAGCCACTTTATCAGCA | 59.85 | 60.00 | 254 | 50 |
100.00%
|
61.54%
|
| Primer 585 | Clostridioides | ACATGACTGCGCAAGGCT | TGGCTCTTGCAAGCTTTACA | 59.97 | 58.02 | 248 | 50 |
100.00%
|
61.54%
|
| Primer 586 | Clostridioides | ACATGACTGCGCAAGGCT | GCATTATTGGCTCTTGCAAGC | 59.97 | 58.80 | 255 | 50 |
100.00%
|
61.54%
|
| Primer 587 | Clostridioides | ACATGACTGCGCAAGGCT | TTGGCTCTTGCAAGCTTTACA | 59.97 | 58.69 | 249 | 50 |
100.00%
|
61.54%
|
| Primer 588 | Clostridioides | GGCCAAATGGGGAACTTGC | TTGAACCAAGACCTGCAGCT | 59.70 | 59.82 | 195 | 50 |
100.00%
|
69.23%
|
| Primer 589 | Clostridioides | ACCTAGAAGTGGGCTTGCC | AGCCAAACCCACCTTTTCCT | 59.31 | 59.74 | 236 | 50 |
100.00%
|
61.54%
|
| Primer 590 | Clostridioides | ATGTTAAGCGCAACACAGGT | CACCCTCCAGAGCAATCCA | 58.40 | 59.01 | 238 | 50 |
100.00%
|
61.54%
|