| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 601 | Clostridioides | GACACAAAATGGCCAGATGGT | TGAGCCACCTGAAACAAACAC | 59.10 | 59.25 | 208 | 50 |
100.00%
|
69.23%
|
| Primer 602 | Clostridioides | GACACAAAATGGCCAGATGGT | GTGAGCCACCTGAAACAAACA | 59.10 | 59.25 | 209 | 50 |
100.00%
|
69.23%
|
| Primer 603 | Clostridioides | TTGTCTGCATGGCCAGGT | AGCAATCCACAATGTTGGTGT | 59.15 | 58.68 | 186 | 50 |
100.00%
|
69.23%
|
| Primer 604 | Clostridioides | GGAATACACCTCCCCTTGCT | GCTGCTACAAGTTCTGCCC | 59.08 | 58.82 | 298 | 50 |
100.00%
|
61.54%
|
| Primer 605 | Clostridioides | GGAATACACCTCCCCTTGCT | AGCTGCTACAAGTTCTGCCC | 59.08 | 60.32 | 299 | 50 |
100.00%
|
61.54%
|
| Primer 606 | Clostridioides | GGAAGGCAGAACTCCGGTT | TTCATCTGCCCAAGTTGCTT | 59.62 | 58.00 | 181 | 50 |
100.00%
|
61.54%
|
| Primer 607 | Clostridioides | GGAAGGCAGAACTCCGGTT | TTTCATCTGCCCAAGTTGCT | 59.62 | 58.00 | 182 | 50 |
100.00%
|
61.54%
|
| Primer 608 | Clostridioides | TGTTGAAGAAGGATGGGCAAA | TGCTCTCCAACTTCTTCACCT | 58.04 | 58.95 | 153 | 50 |
100.00%
|
69.23%
|
| Primer 609 | Clostridioides | TTGTTGAAGAAGGATGGGCAA | TGCTCTCCAACTTCTTCACCT | 58.04 | 58.95 | 154 | 50 |
100.00%
|
69.23%
|
| Primer 610 | Clostridioides | AACTGTTGAGGCTCATTGGC | GCTTCCACATGGGCATGAA | 58.75 | 58.42 | 154 | 50 |
100.00%
|
69.23%
|