| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 621 | Clostridioides | TGCAATTGGTACAGCACATGG | GCCGACTTAACATCACTCGAA | 59.45 | 58.40 | 198 | 50 |
100.00%
|
61.54%
|
| Primer 622 | Clostridioides | GCAATTGGTACAGCACATGGT | GCCGACTTAACATCACTCGAA | 59.45 | 58.40 | 197 | 50 |
100.00%
|
61.54%
|
| Primer 623 | Clostridioides | TGGGTAATGCAGGGCAAACT | GCTACGGCATTGGCTACATC | 59.89 | 59.13 | 232 | 49 |
100.00%
|
61.54%
|
| Primer 624 | Clostridioides | ACGTTGTGCAGGTGGAGT | AGTATCGCCAGCCACATCT | 59.09 | 58.48 | 184 | 49 |
100.00%
|
69.23%
|
| Primer 625 | Clostridioides | AGACGTTGTGCAGGTGGAG | AGTATCGCCAGCCACATCT | 59.93 | 58.48 | 186 | 49 |
100.00%
|
69.23%
|
| Primer 626 | Clostridioides | AGACGTTGTGCAGGTGGA | AGTATCGCCAGCCACATCT | 58.77 | 58.48 | 186 | 49 |
100.00%
|
69.23%
|
| Primer 627 | Clostridioides | GACGTTGTGCAGGTGGAGT | AGTATCGCCAGCCACATCT | 60.23 | 58.48 | 185 | 49 |
100.00%
|
69.23%
|
| Primer 628 | Clostridioides | GCAGTGAAGCGAGAGATGC | TTGCCCAACAAGACTGGTCA | 58.99 | 59.74 | 237 | 49 |
100.00%
|
46.15%
|
| Primer 629 | Clostridioides | GGTGGTACTGGAGGAGTTGC | CTCCAGCCCATCTTGGTCT | 60.04 | 58.69 | 193 | 49 |
100.00%
|
69.23%
|
| Primer 630 | Clostridioides | GGGGAGGAAGTGCCATTGT | TGAGGTTTCACTCCAAGCTCT | 59.62 | 58.95 | 196 | 49 |
100.00%
|
61.54%
|