| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 631 | Clostridioides | AGGTGCTGGTGCTGTTGT | GAACCAGCAACAACGGCA | 59.40 | 58.88 | 189 | 49 |
100.00%
|
69.23%
|
| Primer 632 | Clostridioides | GACCCCTCAGAAGGTGAACT | TTCCTGATGCTCCTGCTTCT | 58.65 | 58.72 | 172 | 49 |
100.00%
|
61.54%
|
| Primer 633 | Clostridioides | AGACCCCTCAGAAGGTGAACT | ATTCCTGATGCTCCTGCTTCT | 60.13 | 59.15 | 174 | 49 |
100.00%
|
61.54%
|
| Primer 634 | Clostridioides | TGCAGGCCTTGGCATACT | CTGGTCCCATCAAGCCTGT | 58.91 | 59.31 | 225 | 49 |
100.00%
|
61.54%
|
| Primer 635 | Clostridioides | TGCAGGCCTTGGCATACT | ACTGGTCCCATCAAGCCTG | 58.91 | 59.31 | 226 | 49 |
100.00%
|
61.54%
|
| Primer 636 | Clostridioides | TGCACCACTGCTTGCACA | TCAGTTCCCTTTCCCGACA | 60.44 | 58.16 | 267 | 48 |
100.00%
|
61.54%
|
| Primer 637 | Clostridioides | TGCACCACTGCTTGCACA | TTCAGTTCCCTTTCCCGACA | 60.44 | 58.87 | 268 | 48 |
100.00%
|
61.54%
|
| Primer 638 | Clostridioides | TGCACCACTGCTTGCACA | TCAGTTCCCTTTCCCGACAT | 60.44 | 58.64 | 267 | 48 |
100.00%
|
61.54%
|
| Primer 639 | Clostridioides | TCAGGAAAGCAGTGAAGCGA | TGCCCAACAAGACTGGTCA | 59.61 | 59.08 | 244 | 48 |
100.00%
|
46.15%
|
| Primer 640 | Clostridioides | AGACACAAAATGGCCAGATGG | TCAAGTGAGCCACCTGAAACA | 58.83 | 59.79 | 214 | 48 |
100.00%
|
69.23%
|