| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 661 | Clostridioides | ATGGAAGGCAGAACTCCGG | TCATCTGCCCAAGTTGCTTTG | 59.40 | 59.39 | 182 | 47 |
100.00%
|
61.54%
|
| Primer 662 | Clostridioides | ATGGAAGGCAGAACTCCGG | ATCTGCCCAAGTTGCTTTGT | 59.40 | 58.29 | 180 | 47 |
100.00%
|
61.54%
|
| Primer 663 | Clostridioides | AAGGGGATAGACTGGATGCAA | CCATCACAGACTCACTCGCTT | 58.51 | 60.07 | 271 | 47 |
100.00%
|
61.54%
|
| Primer 664 | Clostridioides | GACCCCTCAGAAGGTGAACT | TCCTGATGCTCCTGCTTCTT | 58.65 | 58.72 | 171 | 47 |
100.00%
|
61.54%
|
| Primer 665 | Clostridioides | AGACCCCTCAGAAGGTGAACT | TTCCTGATGCTCCTGCTTCTT | 60.13 | 59.37 | 173 | 47 |
100.00%
|
61.54%
|
| Primer 666 | Clostridioides | CACGAAGGAGAAGAATGTGGC | GGTGGAGTCATTGCCCAGA | 59.27 | 59.31 | 173 | 47 |
100.00%
|
61.54%
|
| Primer 667 | Clostridioides | AGCAGCAGCAGGATGTAACTT | GCACTCTTTCTCCATCCCCA | 60.00 | 59.38 | 237 | 47 |
100.00%
|
61.54%
|
| Primer 668 | Clostridioides | GCAGGTACCAGTGGGAGGAT | CTGCACACCCCAGTTCCTT | 60.98 | 59.85 | 274 | 47 |
100.00%
|
61.54%
|
| Primer 669 | Clostridioides | GCAGGTACCAGTGGGAGGAT | TGCACACCCCAGTTCCTT | 60.98 | 58.66 | 273 | 47 |
100.00%
|
53.85%
|
| Primer 670 | Clostridioides | GGTACCAGTGGGAGGATAGGA | CTGCACACCCCAGTTCCTT | 59.78 | 59.85 | 271 | 47 |
100.00%
|
38.46%
|