| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 671 | Clostridioides | AGGTACCAGTGGGAGGATAGG | CTGCACACCCCAGTTCCTT | 59.78 | 59.85 | 272 | 47 |
100.00%
|
61.54%
|
| Primer 672 | Clostridioides | GGTACCAGTGGGAGGATAGGA | TGCACACCCCAGTTCCTT | 59.78 | 58.66 | 270 | 47 |
100.00%
|
38.46%
|
| Primer 673 | Clostridioides | AGGTACCAGTGGGAGGATAGG | TGCACACCCCAGTTCCTT | 59.78 | 58.66 | 271 | 47 |
100.00%
|
53.85%
|
| Primer 674 | Clostridioides | TCCACAAAGCCCTCAAGCC | CAGTTTCTTGTGCCAAAGCCA | 60.23 | 59.86 | 230 | 46 |
100.00%
|
53.85%
|
| Primer 675 | Clostridioides | ACTGGCGAAGGTGATGGA | CCACCTTCAAAGCCAGAACC | 58.19 | 59.04 | 296 | 46 |
100.00%
|
69.23%
|
| Primer 676 | Clostridioides | ACCCCAGAAGCAACTAAGGA | ACTGCACCTTTAGGACTTCCA | 58.26 | 58.95 | 170 | 46 |
100.00%
|
69.23%
|
| Primer 677 | Clostridioides | TGCAATTGGTACAGCACATGG | TGCCGACTTAACATCACTCG | 59.45 | 58.01 | 199 | 46 |
100.00%
|
61.54%
|
| Primer 678 | Clostridioides | GCAATTGGTACAGCACATGGT | TGCCGACTTAACATCACTCG | 59.45 | 58.01 | 198 | 46 |
100.00%
|
61.54%
|
| Primer 679 | Clostridioides | TGCAATTGGTACAGCACATGG | ATGCCGACTTAACATCACTCG | 59.45 | 58.46 | 200 | 46 |
100.00%
|
61.54%
|
| Primer 680 | Clostridioides | GCAATTGGTACAGCACATGGT | ATGCCGACTTAACATCACTCG | 59.45 | 58.46 | 199 | 46 |
100.00%
|
61.54%
|