| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Dehalococcoides | GGCTTGGTGGAAACCGGTA | TGCGTTCCTGCCCAAACA | 59.93 | 60.12 | 226 | 133 |
100.00%
|
400.00%
|
| Primer 2 | Dehalococcoides | ACCCCAAGAGCGGTACTGT | AGCACCGGTTGGCTTTGA | 60.54 | 59.81 | 203 | 132 |
100.00%
|
100.00%
|
| Primer 3 | Dehalococcoides | TAGGCTTGGTGGAAACCGG | TGCGTTCCTGCCCAAACA | 59.62 | 60.12 | 228 | 130 |
100.00%
|
200.00%
|
| Primer 4 | Dehalococcoides | ATGCCTAACCCCAAGAGCG | AGCACCGGTTGGCTTTGA | 59.78 | 59.81 | 210 | 124 |
100.00%
|
100.00%
|
| Primer 5 | Dehalococcoides | CGCCAAATCGTTAGCTGACG | AATGAGGGTGCGCATTGC | 59.97 | 59.11 | 154 | 121 |
100.00%
|
400.00%
|
| Primer 6 | Dehalococcoides | CGCCAAATCGTTAGCTGACG | CGAATGAGGGTGCGCATTG | 59.97 | 59.94 | 156 | 121 |
100.00%
|
400.00%
|
| Primer 7 | Dehalococcoides | CGCCAAATCGTTAGCTGACG | GAATGAGGGTGCGCATTGC | 59.97 | 60.23 | 155 | 121 |
100.00%
|
400.00%
|
| Primer 8 | Dehalococcoides | TGTCCTTCCGCACAGCAA | ACCAACCGCTATAGCAACCA | 59.49 | 59.38 | 153 | 119 |
100.00%
|
200.00%
|
| Primer 9 | Dehalococcoides | ACGGCAAAACAGACGGCA | ACTGGTGCTTGCTGGCAT | 60.52 | 59.88 | 171 | 116 |
100.00%
|
400.00%
|
| Primer 10 | Dehalococcoides | TGACGAAGGTGGTGCTTCC | TTTGCAAAGCAGCTCGCC | 59.93 | 59.66 | 193 | 116 |
100.00%
|
400.00%
|