| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Enterobacter | TTTCCACCTCGGGAATGGC | CCCTTACGTGCGTCTTTGC | 60.00 | 59.50 | 172 | 133 |
100.00%
|
30.77%
|
| Primer 2 | Enterobacter | TTTCCACCTCGGGAATGGC | GCCCTTACGTGCGTCTTTG | 60.00 | 59.50 | 173 | 133 |
100.00%
|
30.77%
|
| Primer 3 | Enterobacter | TGACTACAACGAGGCCTGC | TGCTGAGGAGGAATGGGGA | 59.71 | 59.92 | 186 | 130 |
100.00%
|
38.46%
|
| Primer 4 | Enterobacter | TTTCCACCTCGGGAATGGC | CCTTACGTGCGTCTTTGCC | 60.00 | 59.50 | 171 | 128 |
100.00%
|
30.77%
|
| Primer 5 | Enterobacter | TCAATTCCCTTGAGAGCCTGG | TACGGACTTCGGCACTTCC | 59.72 | 59.41 | 161 | 127 |
100.00%
|
30.77%
|
| Primer 6 | Enterobacter | AGCCGTGCTGCTGATTGT | CAGCGTGGTGTGCAATGT | 59.97 | 58.97 | 220 | 123 |
100.00%
|
30.77%
|
| Primer 7 | Enterobacter | GCGAGGTTGAGGTGGATGT | ATCAAGCTCGAAGGCCTCC | 59.70 | 59.48 | 155 | 123 |
100.00%
|
30.77%
|
| Primer 8 | Enterobacter | CGACACCATCAAACGCGAG | ATCAAGCTCGAAGGCCTCC | 59.58 | 59.48 | 169 | 123 |
100.00%
|
38.46%
|
| Primer 9 | Enterobacter | AGAGGCCTGCGATCTGTTG | TGCTGAGGAGGAATCGGGA | 59.78 | 60.00 | 177 | 122 |
100.00%
|
46.15%
|
| Primer 10 | Enterobacter | CGATCTGTTGCGTTCAGGC | TGCTGAGGAGGAATCGGGA | 59.58 | 60.00 | 168 | 122 |
100.00%
|
46.15%
|