| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Enterobacter | TGACGCTGCAACACCTGT | TACGCACAATCGGCAGCA | 59.81 | 60.05 | 258 | 113 |
100.00%
|
30.77%
|
| Primer 22 | Enterobacter | AGCTATTGCGGTGGCGTT | AGCTAAACGGAGCCTGCT | 60.05 | 58.61 | 269 | 112 |
100.00%
|
38.46%
|
| Primer 23 | Enterobacter | AGCTATTGCGGTGGCGTT | ACGGAGCCTGCTTACCTTC | 60.05 | 59.40 | 263 | 112 |
100.00%
|
38.46%
|
| Primer 24 | Enterobacter | TGCGTTCAGGCTACGTGA | TGCTGAGGAGGAATGGGGA | 58.96 | 59.92 | 160 | 112 |
100.00%
|
38.46%
|
| Primer 25 | Enterobacter | TTGCGTTCAGGCTACGTGA | TGCTGAGGAGGAATGGGGA | 59.64 | 59.92 | 161 | 112 |
100.00%
|
46.15%
|
| Primer 26 | Enterobacter | CTGTTGCGTTCAGGCTACG | TGCTGAGGAGGAATGGGGA | 59.22 | 59.92 | 164 | 112 |
100.00%
|
46.15%
|
| Primer 27 | Enterobacter | TGGCCATGCGTTTCGGTA | AACCGGAGCCTGATCGTT | 59.65 | 58.61 | 278 | 112 |
100.00%
|
30.77%
|
| Primer 28 | Enterobacter | TGCGTTCAGGCTACGTCAA | TGCTGAGGAGGAATCGGGA | 59.64 | 60.00 | 160 | 112 |
100.00%
|
46.15%
|
| Primer 29 | Enterobacter | GCAGCGGTTTTGCCTCAA | ATTCGGTTGCCTGCCAGT | 59.27 | 59.56 | 213 | 112 |
100.00%
|
38.46%
|
| Primer 30 | Enterobacter | TTTCCACCTCGGGAATGGC | ACGTGCGTCTTTGCCTTT | 60.00 | 58.18 | 167 | 110 |
100.00%
|
30.77%
|