| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Fusobacterium | AGTGGAGCGGTAGTAGCAG | TTCTATCCCTACACCAGCTGG | 58.51 | 58.60 | 204 | 181 |
100.00%
|
15.15%
|
| Primer 2 | Fusobacterium | GCCCTTGCTCCAATACTTGT | TCCAAGCCCTTCAAAGCCTC | 58.16 | 60.25 | 297 | 148 |
100.00%
|
12.12%
|
| Primer 3 | Fusobacterium | GCCCTTGCTCCAATACTTGT | CCCTTCAAAGCCTCCAAGC | 58.16 | 58.73 | 291 | 148 |
100.00%
|
12.12%
|
| Primer 4 | Fusobacterium | GCCCTTGCTCCAATACTTGT | AGCCCTTCAAAGCCTCCAAG | 58.16 | 60.25 | 293 | 140 |
100.00%
|
12.12%
|
| Primer 5 | Fusobacterium | GCCCTTGCTCCAATACTTGT | CCTTCAAAGCCTCCAAGCC | 58.16 | 58.73 | 290 | 138 |
100.00%
|
18.18%
|
| Primer 6 | Fusobacterium | AGGGCTTTGTCCTACACTTGG | ACTTTCTGCTCTTCCAAGCAC | 59.93 | 58.77 | 283 | 136 |
100.00%
|
21.21%
|
| Primer 7 | Fusobacterium | GGGCTTTGTCCTACACTTGG | ACTTTCTGCTCTTCCAAGCAC | 58.47 | 58.77 | 282 | 136 |
100.00%
|
21.21%
|
| Primer 8 | Fusobacterium | GCCCTTGCTCCAATACTTGT | AAGCCCTTCAAAGCCTCCA | 58.16 | 59.15 | 294 | 135 |
100.00%
|
12.12%
|
| Primer 9 | Fusobacterium | AGCGGTAGTAGCAGGTCCA | TTCTATCCCTACACCAGCTGG | 60.00 | 58.60 | 199 | 135 |
100.00%
|
15.15%
|
| Primer 10 | Fusobacterium | GTTGGAATTGCAGGGCAGG | GCTACTGCAACTTGCCCTTG | 59.41 | 59.76 | 248 | 131 |
100.00%
|
12.12%
|