| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Fusobacterium | GCGGTAGTAGCAGGTCCAAT | TTCTATCCCTACACCAGCTGG | 59.54 | 58.60 | 198 | 128 |
100.00%
|
15.15%
|
| Primer 22 | Fusobacterium | TTTGGCGTCGTAGCTTTGAT | ACTGTTTCCGTGAGCAGCA | 58.20 | 59.85 | 221 | 127 |
100.00%
|
15.15%
|
| Primer 23 | Fusobacterium | TGACTTCGACCCACAAGCA | TCCCCAAGGGTCAGTAGCA | 59.17 | 59.84 | 256 | 127 |
100.00%
|
12.12%
|
| Primer 24 | Fusobacterium | ACTTCGACCCACAAGCAGT | TCCCCAAGGGTCAGTAGCA | 59.17 | 59.84 | 254 | 127 |
100.00%
|
12.12%
|
| Primer 25 | Fusobacterium | TGACTTTGACCCACAAGCAG | TTCCCCAAGGGTCAGTTGC | 58.32 | 59.85 | 257 | 124 |
100.00%
|
21.21%
|
| Primer 26 | Fusobacterium | TGTTGACTTTGACCCACAAGC | TTCCCCAAGGGTCAGTTGC | 59.25 | 59.85 | 260 | 124 |
100.00%
|
21.21%
|
| Primer 27 | Fusobacterium | TGGAGTTGGTTCTGCAGCA | GTCTTCCAACACCAGGCCA | 59.47 | 59.85 | 242 | 124 |
100.00%
|
15.15%
|
| Primer 28 | Fusobacterium | GCCCTTGCTCCAATACTTGT | TCCAAGCCCTTCAAAGCCT | 58.16 | 59.15 | 297 | 122 |
100.00%
|
12.12%
|
| Primer 29 | Fusobacterium | GCCCTTGCTCCAATACTTGT | CTCCAAGCCCTTCAAAGCCT | 58.16 | 60.25 | 298 | 122 |
100.00%
|
12.12%
|
| Primer 30 | Fusobacterium | AGGCAGCAGCAGGTTTAGT | GCTACTGCAACTTGCCCTTG | 59.24 | 59.76 | 232 | 121 |
100.00%
|
18.18%
|