| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Gracilibacillus | CCCGACACATAGTGGTGAGG | TGCTGGTCACTAGCTTCAGG | 59.82 | 59.39 | 180 | 189 |
100.00%
|
50.00%
|
| Primer 2 | Gracilibacillus | GGGTCACCTGCAGATAGTGG | TCGTACTTGTCATCCCGTGC | 59.82 | 60.11 | 160 | 168 |
100.00%
|
50.00%
|
| Primer 3 | Gracilibacillus | GGGTCACCTGCAGATAGTGG | CGTACTTGTCATCCCGTGC | 59.82 | 58.63 | 159 | 168 |
100.00%
|
50.00%
|
| Primer 4 | Gracilibacillus | TGCCAGTAGAACACCCAACC | CCTTCGCCTTATCTACCGAGG | 59.89 | 59.73 | 165 | 160 |
100.00%
|
50.00%
|
| Primer 5 | Gracilibacillus | GGCGTGCTTAATAATGGCAGG | CACCGATCAACAAGTAGGTGG | 60.00 | 58.65 | 230 | 156 |
100.00%
|
50.00%
|
| Primer 6 | Gracilibacillus | CCCGACACATAGTGGTGAGG | ATGCTGGTCACTAGCTTCAGG | 59.82 | 59.79 | 181 | 156 |
100.00%
|
50.00%
|
| Primer 7 | Gracilibacillus | GTCACATGCGTGTCGAAGC | TGGCGTTGCTCCAATTTCC | 59.87 | 59.04 | 208 | 154 |
100.00%
|
50.00%
|
| Primer 8 | Gracilibacillus | GCTGGACGCGAAGATCTTG | TCACTCGTGAGCCTTCTGC | 59.00 | 59.71 | 208 | 153 |
100.00%
|
50.00%
|
| Primer 9 | Gracilibacillus | AGAAGCTGGACGCGAAGATC | TCACTCGTGAGCCTTCTGC | 60.18 | 59.71 | 212 | 151 |
100.00%
|
50.00%
|
| Primer 10 | Gracilibacillus | AGAACCTGTTGCCGAGTGG | AGTGGCTGCTAAATGACCTCC | 59.93 | 60.07 | 208 | 149 |
100.00%
|
50.00%
|