| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Halothiobacillus | AGGCTCGCAATGTGCAGT | CGACTTGCGGTTCGATTGC | 59.97 | 60.23 | 225 | 118 |
100.00%
|
100.00%
|
| Primer 2 | Halothiobacillus | AGGCTCGCAATGTGCAGT | GCGACTTGCGGTTCGATTG | 59.97 | 60.23 | 226 | 118 |
100.00%
|
100.00%
|
| Primer 3 | Halothiobacillus | ACGGCACAGAAAACCCCAT | TGCCGTTGACGAGCACAT | 59.85 | 59.97 | 174 | 116 |
100.00%
|
100.00%
|
| Primer 4 | Halothiobacillus | GGCAAATCGAACGGCACAG | TGCCGTTGACGAGCACAT | 60.15 | 59.97 | 184 | 116 |
100.00%
|
100.00%
|
| Primer 5 | Halothiobacillus | TCCATGGACGATTGGGCTG | TGCCGTTGACGAGCACAT | 59.78 | 59.97 | 289 | 116 |
100.00%
|
100.00%
|
| Primer 6 | Halothiobacillus | TCTGCCGTGATGCTGCAT | TCGGTGTGTACCTTGCTGC | 59.73 | 60.30 | 218 | 116 |
100.00%
|
100.00%
|
| Primer 7 | Halothiobacillus | TCTGCCGTGATGCTGCAT | CTTGCTGCCGTGGGTAAAC | 59.73 | 59.42 | 207 | 116 |
100.00%
|
100.00%
|
| Primer 8 | Halothiobacillus | TGTGATGGCGATCCCGTTC | TCGAGAATGCGTGCCGAT | 60.15 | 59.51 | 176 | 116 |
100.00%
|
100.00%
|
| Primer 9 | Halothiobacillus | AACGGACGCAGACAGATCC | TTTGGCTTCGGCTTGCTG | 59.78 | 58.96 | 203 | 115 |
100.00%
|
100.00%
|
| Primer 10 | Halothiobacillus | AGGCTCGCAATGTGCAGT | CCATTTTCACCGCTTGCCC | 59.97 | 60.08 | 249 | 115 |
100.00%
|
100.00%
|