| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Holdemania | AGAGATGTCTGGCGTCAGC | TCAGCCCATGACTTCGTTTTC | 59.48 | 58.85 | 184 | 153 |
100.00%
|
50.00%
|
| Primer 2 | Holdemania | TCGACGTTTCTGCGGTGT | TCAGCGAGTGTTCCACGAC | 59.59 | 60.01 | 256 | 141 |
100.00%
|
50.00%
|
| Primer 3 | Holdemania | GGCTTTGCTTCTGCTCTGC | ATGGCGATCTGAGTGGTGC | 59.79 | 60.15 | 188 | 138 |
100.00%
|
50.00%
|
| Primer 4 | Holdemania | TCGACGTTTCTGCGGTGT | AGCTTCCTTCTGCGGAACC | 59.59 | 60.00 | 219 | 133 |
100.00%
|
100.00%
|
| Primer 5 | Holdemania | TGCAGCCAACGTGGAAGT | TGCGGATCATACAGACGGC | 59.81 | 59.93 | 156 | 133 |
100.00%
|
50.00%
|
| Primer 6 | Holdemania | TCAGGCCTGCGATTGAAGG | TGACGCAAACAGCACTGC | 60.08 | 59.28 | 272 | 128 |
100.00%
|
50.00%
|
| Primer 7 | Holdemania | TGTGCGTGCTGTCCTTGT | TTCCTGCCATTCGCCTGT | 59.81 | 59.24 | 239 | 128 |
100.00%
|
100.00%
|
| Primer 8 | Holdemania | TGTGCGTGCTGTCCTTGT | TCGCCTGTCAGCTGTTGT | 59.81 | 59.18 | 229 | 128 |
100.00%
|
50.00%
|
| Primer 9 | Holdemania | ATGCGTGCCGTTGATTGC | TAACCAGACGCGTCAGCAG | 59.82 | 60.08 | 167 | 128 |
100.00%
|
50.00%
|
| Primer 10 | Holdemania | TGCAGCCAACGTGGAAGT | GCGGATCATACAGACGGCA | 59.81 | 59.93 | 155 | 128 |
100.00%
|
50.00%
|