| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Holdemania | ATCCGACGCTGCACAACA | GCGCTTGTAGTCCTCGTCA | 59.97 | 59.79 | 227 | 122 |
100.00%
|
100.00%
|
| Primer 22 | Holdemania | ACGAATTGGCGTCGCTGA | TCCGCAATACCGCTTTGC | 60.05 | 58.82 | 267 | 122 |
100.00%
|
50.00%
|
| Primer 23 | Holdemania | ACGAATTGGCGTCGCTGA | CTGTCGGCAGCACAAACTG | 60.05 | 59.72 | 207 | 122 |
100.00%
|
50.00%
|
| Primer 24 | Holdemania | ACGAATTGGCGTCGCTGA | TGTCGGCAGCACAAACTG | 60.05 | 58.58 | 206 | 122 |
100.00%
|
50.00%
|
| Primer 25 | Holdemania | TCAGCTGGCGATTGCTTCA | TGGCATGCAGCTGCTTCA | 60.00 | 60.28 | 212 | 122 |
100.00%
|
50.00%
|
| Primer 26 | Holdemania | TGCAGGATCAACGGCTGA | TTCCCGTTGCAAGCGTCA | 58.93 | 60.20 | 192 | 122 |
100.00%
|
50.00%
|
| Primer 27 | Holdemania | TGCAGGATCAACGGCTGAG | TTCCCGTTGCAAGCGTCA | 60.08 | 60.20 | 192 | 122 |
100.00%
|
50.00%
|
| Primer 28 | Holdemania | GCTTTGTGCGTCAGGAAGC | TTCCCGTTGCAAGCGTCA | 60.08 | 60.20 | 264 | 122 |
100.00%
|
50.00%
|
| Primer 29 | Holdemania | TGCAGCCAACGTGGAAGT | TGCTTGGAGCCGTTGTTCA | 59.81 | 60.15 | 244 | 122 |
100.00%
|
50.00%
|
| Primer 30 | Holdemania | AAGAAGAACGCGTGCGGA | CGCCATTGTTCAGCGCAA | 59.97 | 59.75 | 279 | 120 |
100.00%
|
50.00%
|