| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Klebsiella | GCTGGCCTGCAGAAAATGG | TCGCCTGCGAATACCACTG | 59.78 | 60.15 | 163 | 158 |
100.00%
|
45.45%
|
| Primer 2 | Klebsiella | AACGCAAATGGCTGCAGC | GCCCAAACAGCGCAATACC | 60.05 | 60.15 | 156 | 154 |
100.00%
|
45.45%
|
| Primer 3 | Klebsiella | GCAGAAAATGGCCACGGTG | TCGCCTGCGAATACCACTG | 60.08 | 60.15 | 155 | 146 |
100.00%
|
54.55%
|
| Primer 4 | Klebsiella | GCCTGCAGAAAATGGCCAC | TCGCCTGCGAATACCACTG | 60.08 | 60.15 | 159 | 142 |
100.00%
|
45.45%
|
| Primer 5 | Klebsiella | AAATCGCGTCGCCAATGC | TCAGGTAACGGCAGTGTGG | 59.90 | 59.63 | 156 | 141 |
100.00%
|
36.36%
|
| Primer 6 | Klebsiella | ACTCGAAATGACTGCGTGC | GCGCAGGCGATTGATCAT | 58.85 | 58.66 | 201 | 139 |
100.00%
|
36.36%
|
| Primer 7 | Klebsiella | CACTCGAAATGACTGCGTGC | GCGCAGGCGATTGATCAT | 60.18 | 58.66 | 202 | 139 |
100.00%
|
36.36%
|
| Primer 8 | Klebsiella | AATTGCTCTCCCTCGGCA | TGTGCAACCGCTTCCAGT | 58.60 | 59.81 | 151 | 138 |
100.00%
|
45.45%
|
| Primer 9 | Klebsiella | AAATTGCTCTCCCTCGGCA | TGTGCAACCGCTTCCAGT | 59.31 | 59.81 | 152 | 138 |
100.00%
|
45.45%
|
| Primer 10 | Klebsiella | TCAGGAAACCACACCGCA | TCTTGTGGCACTGCTGCA | 59.09 | 59.81 | 182 | 138 |
100.00%
|
36.36%
|