| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Klebsiella | TGCCATCATTGCGACGGA | ACCGCAGCGTCGATATCA | 59.73 | 58.88 | 240 | 132 |
100.00%
|
36.36%
|
| Primer 22 | Klebsiella | TGCCATCATTGCGACGGA | CACCGCAGCGTCGATATCA | 59.73 | 60.30 | 241 | 132 |
100.00%
|
36.36%
|
| Primer 23 | Klebsiella | CCCCTTCGACAATCCGCAA | TCAGTGTCGACCAGTTGGC | 60.38 | 59.93 | 167 | 130 |
100.00%
|
36.36%
|
| Primer 24 | Klebsiella | AAGTCGTTCCGCAGCGAT | CTTGAAGGTCTGCTCGGCA | 59.74 | 60.00 | 291 | 128 |
100.00%
|
72.73%
|
| Primer 25 | Klebsiella | AAGTCGTTCCGCAGCGAT | TTGAAGGTCTGCTCGGCA | 59.74 | 58.85 | 290 | 128 |
100.00%
|
72.73%
|
| Primer 26 | Klebsiella | CTCGAAATGACTGCGTGCA | GCGCAGGCGATTGATCAT | 58.85 | 58.66 | 200 | 128 |
100.00%
|
36.36%
|
| Primer 27 | Klebsiella | ACTCGAAATGACTGCGTGCA | GCGCAGGCGATTGATCAT | 60.60 | 58.66 | 201 | 128 |
100.00%
|
36.36%
|
| Primer 28 | Klebsiella | ATCGACAACCGCTTCGCT | GGCTTCGCTTTTCCACAGC | 59.74 | 60.08 | 291 | 128 |
100.00%
|
45.45%
|
| Primer 29 | Klebsiella | ATCGACAACCGCTTCGCT | TGGGGTCGAAAGAGAGGGT | 59.74 | 59.84 | 259 | 128 |
100.00%
|
45.45%
|
| Primer 30 | Klebsiella | ATCGACAACCGCTTCGCT | CGGCTTCGCTTTTCCACAG | 59.74 | 59.79 | 292 | 128 |
100.00%
|
45.45%
|