| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Kluyvera | CGTCTTCGCAAAGTCGCTG | TTTTCACGCGTTCGGCAC | 59.87 | 59.67 | 289 | 147 |
100.00%
|
50.00%
|
| Primer 2 | Kluyvera | GTCTTCGCAAAGTCGCTGC | TTTTCACGCGTTCGGCAC | 60.15 | 59.67 | 288 | 146 |
100.00%
|
50.00%
|
| Primer 3 | Kluyvera | TCTTCGCAAAGTCGCTGC | TTTTCACGCGTTCGGCAC | 58.76 | 59.67 | 287 | 146 |
100.00%
|
50.00%
|
| Primer 4 | Kluyvera | TGATGCAGGTGAACGCCA | AGACGGCATCACTTCGGTC | 59.57 | 59.78 | 239 | 135 |
100.00%
|
50.00%
|
| Primer 5 | Kluyvera | TGATGCAGGTGAACGCCA | ACGGCATCACTTCGGTCA | 59.57 | 58.94 | 237 | 132 |
100.00%
|
50.00%
|
| Primer 6 | Kluyvera | TGATGCAGGTGAACGCCA | GACGGCATCACTTCGGTCA | 59.57 | 60.08 | 238 | 132 |
100.00%
|
100.00%
|
| Primer 7 | Kluyvera | TGCCCTGCAAAGCGATGA | CAGTGATCGGTCAGCGTGA | 59.97 | 59.79 | 156 | 132 |
100.00%
|
100.00%
|
| Primer 8 | Kluyvera | TTTTATGCTGCGCTGCGG | TATCAATGGCAGCCACCGG | 59.52 | 60.15 | 228 | 130 |
100.00%
|
50.00%
|
| Primer 9 | Kluyvera | TCGTCGCCACGCAGAAAT | AACCACCGACATCGCCTT | 60.05 | 59.25 | 197 | 125 |
100.00%
|
50.00%
|
| Primer 10 | Kluyvera | TCACCGTGATTCACCGCA | TGTCGCTTCACGCTTCCA | 59.26 | 59.58 | 155 | 124 |
100.00%
|
50.00%
|