| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Lachnospira | GCACAGAACGCACTTGACAG | ACCGCTCATCTGCTCTTCC | 60.04 | 59.48 | 162 | 114 |
100.00%
|
200.00%
|
| Primer 2 | Lachnospira | TGTGGGCATGTTCAGGTGG | TAGCAGACGCACCAGACTG | 60.23 | 59.41 | 288 | 113 |
100.00%
|
100.00%
|
| Primer 3 | Lachnospira | TCTTCATGGTCACCGCTGG | TGCAGCAATTGTCGCACC | 59.70 | 59.36 | 187 | 110 |
100.00%
|
100.00%
|
| Primer 4 | Lachnospira | TCGGTTGTAGCGGTTGAGG | CGTCTTCAACCTCAACGTGC | 59.71 | 59.77 | 274 | 107 |
100.00%
|
200.00%
|
| Primer 5 | Lachnospira | AGGCACTGGCTTTGAGAGG | AGCTGGTGTTCCGGATACTG | 59.62 | 59.46 | 246 | 102 |
100.00%
|
200.00%
|
| Primer 6 | Lachnospira | TTCGGTTGTAGCGGTTGAGG | CGTCTTCAACCTCAACGTGC | 60.32 | 59.77 | 275 | 101 |
100.00%
|
200.00%
|
| Primer 7 | Lachnospira | TGGCATCAGCAGCGACAT | TACAGCCGTCCTTCGTCAG | 59.73 | 59.12 | 261 | 99 |
100.00%
|
200.00%
|
| Primer 8 | Lachnospira | TGGCATCAGCAGCGACAT | CCACCATTCTCGCCTACTCC | 59.73 | 59.89 | 216 | 99 |
100.00%
|
100.00%
|
| Primer 9 | Lachnospira | CGGACATCAAGAGCGTTGC | TCGCCATCGCTTAGTCTCC | 59.58 | 59.27 | 203 | 96 |
100.00%
|
200.00%
|
| Primer 10 | Lachnospira | TCCGGAGTTTGGCAGACTG | GTGAGTGGCTTTGCTGTGC | 59.63 | 60.01 | 234 | 96 |
100.00%
|
200.00%
|