| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Lachnospira | TGGCATCAGCAGCGACAT | ACAGCCGTCCTTCGTCAGT | 59.73 | 61.20 | 260 | 93 |
100.00%
|
100.00%
|
| Primer 12 | Lachnospira | AGCAACAGGTGGTAAGCAGT | TCGCCTACAACGCTAGCTG | 59.53 | 59.86 | 180 | 93 |
100.00%
|
100.00%
|
| Primer 13 | Lachnospira | AGCAACAGGTGGTAAGCAGT | CGCCTACAACGCTAGCTGT | 59.53 | 60.15 | 179 | 93 |
100.00%
|
100.00%
|
| Primer 14 | Lachnospira | ATGGGCAGGAACAGGAAGC | TCCAACTACCCAGCCAAGC | 60.00 | 59.62 | 211 | 93 |
100.00%
|
100.00%
|
| Primer 15 | Lachnospira | CCGACAACCAGAATGCAGG | ACACAAACCCGATTATGCCAG | 58.83 | 58.91 | 184 | 92 |
100.00%
|
50.00%
|
| Primer 16 | Lachnospira | TGGCATCAGCAGCGACAT | TTCTCGCCTACTCCAGGGA | 59.73 | 59.30 | 210 | 90 |
100.00%
|
50.00%
|
| Primer 17 | Lachnospira | GCAACGGTAAGAGCGATGG | TGCATCAACGCCTTTCTGC | 58.99 | 59.42 | 237 | 90 |
100.00%
|
100.00%
|
| Primer 18 | Lachnospira | TGGATCCACTTGCAGATAAGC | TGCTGTCTTGAACTTGCCC | 58.08 | 58.28 | 188 | 90 |
100.00%
|
100.00%
|
| Primer 19 | Lachnospira | AGCGGATTGTGCTAACGGA | AGCTTCTCGCCACATGCA | 59.41 | 59.65 | 249 | 88 |
100.00%
|
100.00%
|
| Primer 20 | Lachnospira | AGGAAGCACAGAACGCACT | ACCGCTCATCTGCTCTTCC | 59.55 | 59.48 | 167 | 88 |
100.00%
|
100.00%
|