| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Lactobacillus | GCTTGAAGCTGGTGTCCAC | ATACGCCACCGTCTTGTGC | 59.05 | 60.74 | 173 | 146 |
100.00%
|
28.89%
|
| Primer 2 | Lactobacillus | ACCGCAACGATTCTCGCA | ACGGATTGCTTCTGGGACT | 60.05 | 58.62 | 194 | 138 |
100.00%
|
20.00%
|
| Primer 3 | Lactobacillus | ACAAAGTTGCTGAAGGCGAT | CGGTGACCGTACTTGCTGT | 58.39 | 60.01 | 235 | 130 |
100.00%
|
24.44%
|
| Primer 4 | Lactobacillus | AAGCTGGTGTCCACTTCGG | ATACGCCACCGTCTTGTGC | 59.93 | 60.74 | 168 | 130 |
100.00%
|
24.44%
|
| Primer 5 | Lactobacillus | TTCCGTGCTGGTGACACTG | ACACCAACACCGGAAGCA | 60.23 | 59.41 | 152 | 127 |
100.00%
|
24.44%
|
| Primer 6 | Lactobacillus | AGACCGGTGGTAAGCAATACA | CGGTGACCGTACTTGCTGT | 59.10 | 60.01 | 253 | 126 |
100.00%
|
31.11%
|
| Primer 7 | Lactobacillus | ACCGCAACGATTCTCGCA | ACGGATTGCTTCTGGGACTT | 60.05 | 59.31 | 194 | 125 |
100.00%
|
20.00%
|
| Primer 8 | Lactobacillus | GACCGGTGGTAAGCAATACAA | CGGTGACCGTACTTGCTGT | 58.30 | 60.01 | 252 | 119 |
100.00%
|
31.11%
|
| Primer 9 | Lactobacillus | GCAGAAGGCGACAGCGTAT | ACGGTGACCATACTTGCTGT | 60.52 | 59.31 | 228 | 119 |
100.00%
|
20.00%
|
| Primer 10 | Lactobacillus | GCAGAAGGCGACAGCGTAT | TGACGGTGACCATACTTGCT | 60.52 | 59.03 | 230 | 119 |
100.00%
|
20.00%
|