| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Lactobacillus | GCAGAAGGCGACAGCGTAT | TGACGGTGACCATACTTGCTG | 60.52 | 60.34 | 230 | 119 |
100.00%
|
20.00%
|
| Primer 22 | Lactobacillus | TGGCGTGGTGAAACTGGT | CCAATTTCAGCAGCACGCA | 59.41 | 59.71 | 290 | 117 |
100.00%
|
20.00%
|
| Primer 23 | Lactobacillus | AATGGGTCGTGAATGCGGT | TGAGCCACCACGTTGCAT | 60.00 | 59.89 | 262 | 117 |
100.00%
|
24.44%
|
| Primer 24 | Lactobacillus | CCAGCTCCACAAATGCGTG | ACGGCTTTGCTTTCTGCC | 59.79 | 58.96 | 267 | 116 |
100.00%
|
26.67%
|
| Primer 25 | Lactobacillus | CCAGAACCTTGGGCAGCTT | TCCCAACCAACACCAGCA | 60.23 | 58.99 | 251 | 114 |
100.00%
|
46.67%
|
| Primer 26 | Lactobacillus | CCAGCTCCACAAATGCGTG | CGGCTTTGCTTTCTGCCA | 59.79 | 58.96 | 266 | 113 |
100.00%
|
24.44%
|
| Primer 27 | Lactobacillus | GTGTCCACTTCGGTCACCA | ATACGCCACCGTCTTGTGC | 59.56 | 60.74 | 162 | 113 |
100.00%
|
28.89%
|
| Primer 28 | Lactobacillus | TGCTTGAAGCTGGTGTCCA | ATACGCCACCGTCTTGTGC | 59.47 | 60.74 | 174 | 113 |
100.00%
|
28.89%
|
| Primer 29 | Lactobacillus | TGTCCACTTCGGTCACCA | ATACGCCACCGTCTTGTGC | 58.04 | 60.74 | 161 | 113 |
100.00%
|
26.67%
|
| Primer 30 | Lactobacillus | ACTGTTCGCATCGCTCCA | AAGCCATCCCCTTAGCACG | 59.34 | 59.78 | 256 | 112 |
100.00%
|
20.00%
|