| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 4621 | Leptospira | TCCGCTTTGGATCCGGTT | ACGACGGATGCTTGAACGT | 58.93 | 60.01 | 275 | 37 |
100.00%
|
50.00%
|
| Primer 4622 | Leptospira | TTGGACGAGGCGTTAGGTG | ACTCAAATCGGTCGCACGT | 59.71 | 60.01 | 252 | 37 |
100.00%
|
50.00%
|
| Primer 4623 | Leptospira | AGCGCTGTGGAACTTGCA | TCCGGAACCAATCGCGATT | 60.20 | 59.78 | 156 | 37 |
100.00%
|
50.00%
|
| Primer 4624 | Leptospira | ACGCAATTCCAGAAGCCG | GCTCGGCAGGACTTTCCTT | 58.42 | 60.00 | 256 | 37 |
100.00%
|
50.00%
|
| Primer 4625 | Leptospira | CGTGTGGACAACTTTGCGG | TGTGCGAAAGTTCAACCGC | 60.01 | 59.65 | 166 | 37 |
100.00%
|
50.00%
|
| Primer 4626 | Leptospira | CGGAACAAATCGCCTACGC | CCGGTGCGAATGAGATCCA | 59.65 | 59.86 | 196 | 37 |
100.00%
|
50.00%
|
| Primer 4627 | Leptospira | CGGCTACCACGTCGACATT | GCGTAGGCGATTTGTTCCG | 60.15 | 59.65 | 255 | 37 |
100.00%
|
50.00%
|
| Primer 4628 | Leptospira | TGCGAACTCGGAACACGT | GACGGCTCCTTCGGATCAA | 59.59 | 59.48 | 161 | 37 |
100.00%
|
50.00%
|
| Primer 4629 | Leptospira | CCGACTCCAGTGAAAGCGT | TTCGACCGCTTCCGCTTT | 60.01 | 59.97 | 204 | 37 |
100.00%
|
50.00%
|
| Primer 4630 | Leptospira | TTGCGGAACTGCTCGGAA | CCTTGTTCCCGATCGGCTT | 59.58 | 60.08 | 198 | 37 |
100.00%
|
50.00%
|