| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 4641 | Leptospira | AGGGGATGATGCCACGAGA | ATTGACCCTGGGAAGCCTG | 60.38 | 59.31 | 164 | 37 |
100.00%
|
50.00%
|
| Primer 4642 | Leptospira | TCACGCGTTTGTGATCGC | CGGCAATGGACGGTTTTGG | 59.15 | 60.08 | 191 | 37 |
100.00%
|
50.00%
|
| Primer 4643 | Leptospira | GTGCCTGTCAAAGTGGGGA | TCCGACTTCTTCCGAGCTT | 59.85 | 58.35 | 268 | 37 |
100.00%
|
50.00%
|
| Primer 4644 | Leptospira | CCTCTGGCAACGACGAAGA | TCCGCTTCCGATTCCCAA | 59.71 | 58.61 | 185 | 37 |
100.00%
|
50.00%
|
| Primer 4645 | Leptospira | CCTCTGGCAACGACGAAGA | TTCCGCTTCCGATTCCCA | 59.71 | 58.61 | 186 | 37 |
100.00%
|
50.00%
|
| Primer 4646 | Leptospira | CCGTTTTACTCGAGGGACGG | AATCCCCTGCGCTCATCTT | 60.46 | 59.08 | 229 | 37 |
100.00%
|
50.00%
|
| Primer 4647 | Leptospira | TCCTTGGCTACACCCGAGT | TGAACCGGTTGCGTTCGT | 60.23 | 60.20 | 193 | 37 |
100.00%
|
50.00%
|
| Primer 4648 | Leptospira | CGTAGTCAAACCGATCGCG | ATCGTGGGTTCAGGACGT | 59.09 | 58.21 | 254 | 37 |
100.00%
|
50.00%
|
| Primer 4649 | Leptospira | TGGCAATCCGGACGAACA | ACCTCCAAGTCCTCTCCGT | 59.26 | 59.53 | 162 | 37 |
100.00%
|
50.00%
|
| Primer 4650 | Leptospira | TTCCTGAGCTGACGGGAGA | TGCGGTCAGAGCGGAAAT | 59.92 | 59.34 | 218 | 37 |
100.00%
|
50.00%
|