| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Listeria | AGAAAGCCGTGCTCAAGC | CGACTTGTTCGCGAAGAGC | 58.34 | 59.59 | 230 | 176 |
100.00%
|
25.00%
|
| Primer 2 | Listeria | AAGAAAGCCGTGCTCAAGC | CGACTTGTTCGCGAAGAGC | 59.05 | 59.59 | 231 | 176 |
100.00%
|
30.00%
|
| Primer 3 | Listeria | GAAGAAAGCCGTGCTCAAGC | CGACTTGTTCGCGAAGAGC | 60.11 | 59.59 | 232 | 176 |
100.00%
|
30.00%
|
| Primer 4 | Listeria | ACGGAGAAGCCCAAGAAGC | CGCATCTGTTTCGCCTTCC | 60.00 | 59.57 | 197 | 165 |
100.00%
|
15.00%
|
| Primer 5 | Listeria | ACGGAGAAGCCCAAGAAGC | CCGCATCTGTTTCGCCTTC | 60.00 | 59.57 | 198 | 162 |
100.00%
|
15.00%
|
| Primer 6 | Listeria | ATACGTGGGGACTTCGTGC | CCGTCTGGCAAGTGGTCAT | 59.78 | 60.00 | 241 | 149 |
100.00%
|
15.00%
|
| Primer 7 | Listeria | TCGGACCACTGCGTAATGC | ACGGACAGCATTGCGCAT | 60.45 | 60.75 | 200 | 149 |
100.00%
|
15.00%
|
| Primer 8 | Listeria | CGGCTGAAGAAAACCGTGC | ACTTGTTCACGAAGCGCAG | 60.08 | 59.07 | 235 | 146 |
100.00%
|
20.00%
|
| Primer 9 | Listeria | CGGCTGAAGAAAACCGTGC | GACTTGTTCACGAAGCGCAG | 60.08 | 60.11 | 236 | 146 |
100.00%
|
20.00%
|
| Primer 10 | Listeria | AGCTGCAATGGCTGGAGAC | CGCCATTGTTGCGCAAGT | 60.38 | 60.05 | 219 | 141 |
100.00%
|
20.00%
|