| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Listeria | CGACGACCAAAGAAGCAGC | ACCGGTTCGTCATCCAGTG | 59.51 | 59.71 | 238 | 140 |
100.00%
|
20.00%
|
| Primer 12 | Listeria | GCGACGACCAAAGAAGCAG | ACCGGTTCGTCATCCAGTG | 59.51 | 59.71 | 239 | 140 |
100.00%
|
20.00%
|
| Primer 13 | Listeria | AGGCGACGACCAAAGAAGC | ACCGGTTCGTCATCCAGTG | 60.67 | 59.71 | 241 | 140 |
100.00%
|
20.00%
|
| Primer 14 | Listeria | CTGGTGCAACAGCGCAAA | ACGCTCGTGCTAGAACCAC | 59.59 | 60.08 | 298 | 137 |
100.00%
|
15.00%
|
| Primer 15 | Listeria | AGCCAGATTTCGCAGTCCA | CCCGTATTTTGGATGTCGGC | 59.32 | 59.34 | 294 | 135 |
100.00%
|
20.00%
|
| Primer 16 | Listeria | TCTTTGCAGTTGCCGAAGC | TTGTCGCCTTTCATCATTCCA | 59.35 | 58.21 | 172 | 135 |
100.00%
|
20.00%
|
| Primer 17 | Listeria | ATCTTTGCAGTTGCCGAAGC | TTGTCGCCTTTCATCATTCCA | 59.76 | 58.21 | 173 | 135 |
100.00%
|
20.00%
|
| Primer 18 | Listeria | AATCTTTGCAGTTGCCGAAGC | TTGTCGCCTTTCATCATTCCA | 60.34 | 58.21 | 174 | 135 |
100.00%
|
20.00%
|
| Primer 19 | Listeria | CGCAGTCCAAACAGACGAAC | CCCGTATTTTGGATGTCGGC | 59.77 | 59.34 | 284 | 132 |
100.00%
|
25.00%
|
| Primer 20 | Listeria | CGGAAGAAAGCCGTGCTCA | CGACTTGTTCGCGAAGAGC | 60.67 | 59.59 | 234 | 132 |
100.00%
|
25.00%
|