| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Megasphaera | AAACGCAGCCATCAACGC | TCGTGGGTAGCTTCGCCTA | 59.74 | 60.38 | 207 | 84 |
100.00%
|
66.67%
|
| Primer 42 | Megasphaera | ACGCGTCAAGACCGTTGA | GCTGGTGCCAATTACGTCG | 59.59 | 59.58 | 235 | 80 |
100.00%
|
66.67%
|
| Primer 43 | Megasphaera | AGCGCTGCCGAAATGGAT | ACGCCTGCGCTTTCAGT | 60.12 | 59.93 | 172 | 79 |
100.00%
|
66.67%
|
| Primer 44 | Megasphaera | ATTCCGTCGGCACACGTT | TTTCGTCGCGTTCAGGCA | 59.97 | 60.28 | 175 | 78 |
100.00%
|
66.67%
|
| Primer 45 | Megasphaera | AAACGCAGCCATCAACGC | ATGATGAAGCGGTCGTGGG | 59.74 | 60.15 | 219 | 77 |
100.00%
|
66.67%
|
| Primer 46 | Megasphaera | AGCGCTGCCGAAATGGAT | ACGCCTGCGCTTTCAGTT | 60.12 | 60.59 | 172 | 76 |
100.00%
|
66.67%
|
| Primer 47 | Megasphaera | AAAGAAGCAGGCGTACCGG | GCTTCTGCCAGGGTGACAT | 60.38 | 60.00 | 239 | 76 |
100.00%
|
66.67%
|
| Primer 48 | Megasphaera | GGTGCCGTCATTGCCAAA | TCGGCATGACGAGCTTCA | 58.95 | 59.03 | 226 | 76 |
100.00%
|
66.67%
|
| Primer 49 | Megasphaera | GGTGCCGTCATTGCCAAAG | TCGGCATGACGAGCTTCA | 60.08 | 59.03 | 226 | 76 |
100.00%
|
66.67%
|
| Primer 50 | Megasphaera | CATCCCTCACGGCATCGAA | CGGAACAGGTGATGGCGAT | 59.86 | 60.15 | 176 | 72 |
100.00%
|
66.67%
|