| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Megasphaera | TTCCCGAAAGTCAGCGCT | TTGCGGACCTGTTCGACT | 59.26 | 58.87 | 200 | 95 |
100.00%
|
66.67%
|
| Primer 22 | Megasphaera | AGCTTTCTCGTAGCGGTGG | TGCCGTCTTCAAAGCGGT | 59.78 | 59.89 | 153 | 95 |
100.00%
|
66.67%
|
| Primer 23 | Megasphaera | ACGCAAGAACATCAACGGC | CCGCTGACGTTCTGACCTT | 59.43 | 60.01 | 156 | 94 |
100.00%
|
66.67%
|
| Primer 24 | Megasphaera | TCGTTACCAAGCGCTGCA | ATAGCCCTTGCGCTCGATG | 59.97 | 60.30 | 293 | 94 |
100.00%
|
66.67%
|
| Primer 25 | Megasphaera | TTCCCGAAAGTCAGCGCT | TGCGGACCTGTTCGACTT | 59.26 | 58.87 | 199 | 92 |
100.00%
|
66.67%
|
| Primer 26 | Megasphaera | CAGCACGATGAACGAAGCG | TTGCACATGAGCATGCGC | 59.94 | 59.82 | 269 | 91 |
100.00%
|
66.67%
|
| Primer 27 | Megasphaera | GCAGCAATGCTCGACATCC | ACAGCGATCCCCAAACCA | 59.65 | 58.83 | 209 | 91 |
100.00%
|
66.67%
|
| Primer 28 | Megasphaera | ATTCCGTCGGCACACGTT | TTCGTCGCGTTCAGGCAT | 59.97 | 60.05 | 174 | 89 |
100.00%
|
66.67%
|
| Primer 29 | Megasphaera | TGCCGTCGTCATTGCCAT | GCAATGGGCAGGAGGATCA | 60.05 | 59.77 | 177 | 88 |
100.00%
|
66.67%
|
| Primer 30 | Megasphaera | ATGCCGTCGTCATTGCCA | GCAATGGGCAGGAGGATCA | 60.05 | 59.77 | 178 | 88 |
100.00%
|
66.67%
|