| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Megasphaera | AAAGCAGACCGCATCGCT | TGCCTTCGTTCAGAGCCTG | 60.05 | 60.00 | 253 | 106 |
100.00%
|
66.67%
|
| Primer 12 | Megasphaera | AAAGCAGACCGCATCGCT | TCCGGTTTGCCTTCGTTCA | 60.05 | 59.85 | 260 | 106 |
100.00%
|
66.67%
|
| Primer 13 | Megasphaera | TCGTTACCAAGCGCTGCA | GATAGCCCTTGCGCTCGAT | 59.97 | 60.01 | 294 | 105 |
100.00%
|
66.67%
|
| Primer 14 | Megasphaera | TTTTCTGTCCCCTCGTGGC | AAGCCATGGTCGTCAGCA | 59.93 | 59.25 | 273 | 105 |
100.00%
|
66.67%
|
| Primer 15 | Megasphaera | GTGTCCAGGAAGGCGACAA | CCTGACGACGACGGTAGTT | 59.93 | 59.13 | 225 | 100 |
100.00%
|
66.67%
|
| Primer 16 | Megasphaera | AGCGCTGCCGAAATGGAT | ACGCCTGCGCTTTCAGTT | 60.12 | 60.59 | 172 | 100 |
100.00%
|
66.67%
|
| Primer 17 | Megasphaera | GCCTCATTTCCCTGAGCGA | ATGTTGCGGACGATGCCT | 59.78 | 59.73 | 196 | 98 |
100.00%
|
66.67%
|
| Primer 18 | Megasphaera | GAACAGGACGTACTGCCGA | ACGGTTTGACGATGCGGT | 59.42 | 59.97 | 238 | 98 |
100.00%
|
66.67%
|
| Primer 19 | Megasphaera | AGCGCTGCCGAAATGGAT | ACGCCTGCGCTTTCAGT | 60.12 | 59.93 | 172 | 97 |
100.00%
|
66.67%
|
| Primer 20 | Megasphaera | AGCGCTGCCGAAATGGAT | TACGCCTGCGCTTTCAGT | 60.12 | 59.66 | 173 | 95 |
100.00%
|
66.67%
|