| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1231 | Mesorhizobium | GCGCAACATCGAAGTGCA | TGTTGCGCATCGTGTAGC | 59.44 | 58.84 | 299 | 65 |
100.00%
|
50.00%
|
| Primer 1232 | Mesorhizobium | AAGAAGGCTTCGAGCGCA | TGCGGATCTTGCGGAACA | 59.66 | 59.65 | 198 | 64 |
100.00%
|
50.00%
|
| Primer 1233 | Mesorhizobium | ACCGGTCGATTTGGCACA | TCAGTTTGCCGAGCCGAT | 59.57 | 59.34 | 260 | 64 |
100.00%
|
50.00%
|
| Primer 1234 | Mesorhizobium | AAGGCAAGCTGTTTCGCG | GCAGCGCAACCGACAAAA | 59.36 | 59.97 | 228 | 64 |
100.00%
|
50.00%
|
| Primer 1235 | Mesorhizobium | TTCACGCTCGAACCGCAT | AGCTCGATGTCGCCAACA | 60.05 | 59.34 | 167 | 64 |
100.00%
|
50.00%
|
| Primer 1236 | Mesorhizobium | GTCATCCCTCGGTCGTATCG | TTTGCTCCAGCCCAGGATC | 59.76 | 59.70 | 171 | 64 |
100.00%
|
50.00%
|
| Primer 1237 | Mesorhizobium | GTCATCCCTCGGTCGTATCG | CTTTGCTCCAGCCCAGGAT | 59.76 | 59.69 | 172 | 64 |
100.00%
|
50.00%
|
| Primer 1238 | Mesorhizobium | TGTGCAGACCGTGTCGAT | AAGGAGCCGTTGCCGAAA | 58.95 | 59.89 | 180 | 64 |
100.00%
|
50.00%
|
| Primer 1239 | Mesorhizobium | CTGTGCAGACCGTGTCGAT | AAGGAGCCGTTGCCGAAA | 60.08 | 59.89 | 181 | 64 |
100.00%
|
50.00%
|
| Primer 1240 | Mesorhizobium | TGTGCAGACCGTGTCGATC | AAGGAGCCGTTGCCGAAA | 60.08 | 59.89 | 180 | 64 |
100.00%
|
50.00%
|