| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1241 | Mesorhizobium | ACAACCACCATGGCGACA | TCTGGATCAGCTTGCGCA | 59.49 | 59.34 | 207 | 64 |
100.00%
|
50.00%
|
| Primer 1242 | Mesorhizobium | ACAACGCCTTCACCGACA | ACCGCTGACGAAAAGGCA | 59.50 | 59.89 | 194 | 64 |
100.00%
|
50.00%
|
| Primer 1243 | Mesorhizobium | TTCGCGATCGTTGCAACC | TTGCCGACAAACGCCAAC | 59.14 | 59.59 | 245 | 64 |
100.00%
|
50.00%
|
| Primer 1244 | Mesorhizobium | AAGGCGTCGATTGCGTCA | GGTGTCGGAATTGTTGGCG | 60.05 | 59.79 | 272 | 64 |
100.00%
|
50.00%
|
| Primer 1245 | Mesorhizobium | CATTGCCGAGTCCAATGCG | TGGTTGCGTTCGAGCCTT | 59.94 | 59.89 | 177 | 64 |
100.00%
|
50.00%
|
| Primer 1246 | Mesorhizobium | ACGACGAAACGGTTTGCG | CCGAACAGGAAGCCGATGA | 59.38 | 59.78 | 152 | 64 |
100.00%
|
50.00%
|
| Primer 1247 | Mesorhizobium | AGCGTCGTCAATGTGGCA | ACGGCATGGATGATGGTCG | 59.97 | 60.23 | 296 | 64 |
100.00%
|
50.00%
|
| Primer 1248 | Mesorhizobium | AACATGCTGCCCAAGGACA | TGCGCTGCAATTCCTGGA | 59.85 | 59.97 | 259 | 64 |
100.00%
|
50.00%
|
| Primer 1249 | Mesorhizobium | CTTGTCCCCACCGTGGAAA | AGCCAAAGCAGCACCGAT | 59.85 | 59.97 | 165 | 64 |
100.00%
|
50.00%
|
| Primer 1250 | Mesorhizobium | ATGTGAGCCACGCCAACA | ACAGTTCCTTGACGTCGGC | 59.89 | 60.30 | 183 | 64 |
100.00%
|
50.00%
|