| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Methanobrevibacter | GCACTTCCTGTGGCATTGC | CTACCCCTGTTGCCATTGC | 60.08 | 58.81 | 250 | 157 |
100.00%
|
50.00%
|
| Primer 2 | Methanobrevibacter | ATGATGAAACCGGACGCAG | TCCGAGGTCTGCTTCTAAAGG | 58.23 | 59.17 | 279 | 148 |
100.00%
|
50.00%
|
| Primer 3 | Methanobrevibacter | ATGGGCAAAGGTGGAGGTC | TGAGGAACACCTGCACCTG | 59.62 | 59.55 | 188 | 147 |
100.00%
|
50.00%
|
| Primer 4 | Methanobrevibacter | AGACAGAGACCCACAACATGT | CACCGCATGCAGCACAT | 58.95 | 58.71 | 230 | 138 |
100.00%
|
75.00%
|
| Primer 5 | Methanobrevibacter | GCACTTCCTGTGGCATTGC | TGCCTACCCCTGTTGCCAT | 60.08 | 61.24 | 253 | 138 |
100.00%
|
50.00%
|
| Primer 6 | Methanobrevibacter | TCGGTTCGGCATCTGATCC | AGGTGCATTTGGCTCAACC | 59.56 | 58.65 | 269 | 138 |
100.00%
|
50.00%
|
| Primer 7 | Methanobrevibacter | TCGGTTCGGCATCTGATCC | AAGGTGCATTTGGCTCAACC | 59.56 | 59.32 | 270 | 138 |
100.00%
|
50.00%
|
| Primer 8 | Methanobrevibacter | GCACTTCCTGTGGCATTGC | TTGCACTGCCTACCCCTGT | 60.08 | 61.15 | 259 | 137 |
100.00%
|
50.00%
|
| Primer 9 | Methanobrevibacter | TATGGCTACCGGTGCAGGA | TCTTCGCCTTTGTCAACAACA | 60.38 | 58.64 | 213 | 137 |
100.00%
|
50.00%
|
| Primer 10 | Methanobrevibacter | TCCTATGGCTACCGGTGCA | TCTTCGCCTTTGTCAACAACA | 60.38 | 58.64 | 216 | 137 |
100.00%
|
50.00%
|