| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Methanobrevibacter | TGATGTTTCTGGTGATGCGG | AGGAGACACACCTGCAGGA | 58.55 | 60.15 | 193 | 119 |
100.00%
|
50.00%
|
| Primer 42 | Methanobrevibacter | TGGAACCGGAGCAACACA | GCAAGCTCGTGAAGAGGCT | 59.09 | 60.38 | 189 | 117 |
100.00%
|
50.00%
|
| Primer 43 | Methanobrevibacter | ACTTGGGGTGAAATTGATGCA | ACTTCGCCAGCAATTACAGC | 58.39 | 59.20 | 155 | 116 |
100.00%
|
50.00%
|
| Primer 44 | Methanobrevibacter | ACTTGGGGTGAAATTGATGCA | AACTTCGCCAGCAATTACAGC | 58.39 | 59.80 | 156 | 116 |
100.00%
|
50.00%
|
| Primer 45 | Methanobrevibacter | ATGATGAAACCGGACGCAG | AGCAAGAGATGGGGAAGATGT | 58.23 | 58.80 | 198 | 115 |
100.00%
|
50.00%
|
| Primer 46 | Methanobrevibacter | TCGGTTCGGCATCTGATCC | AAAGGTGCATTTGGCTCAACC | 59.56 | 59.93 | 271 | 115 |
100.00%
|
50.00%
|
| Primer 47 | Methanobrevibacter | TGGAACCGGAGCAACACA | AGCAAGCTCGTGAAGAGGC | 59.09 | 60.38 | 190 | 115 |
100.00%
|
50.00%
|
| Primer 48 | Methanobrevibacter | ACCTCAGGGAACTCTTGAGGA | TCTTGGGCTCAGAATTGGGG | 59.85 | 59.67 | 254 | 115 |
100.00%
|
50.00%
|
| Primer 49 | Methanobrevibacter | TCAAACCTCGTCACGCTGT | AAGCCAACAAACGTCCGC | 59.56 | 59.28 | 159 | 114 |
100.00%
|
100.00%
|
| Primer 50 | Methanobrevibacter | GGGCAAAGGTGGAGGTCTT | TGAGGAACACCTGCACCTG | 59.54 | 59.55 | 186 | 114 |
100.00%
|
50.00%
|