| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 51 | Methanobrevibacter | AACAGCTCACAGCCAGCTT | AAGTTGACGTCCGGACCA | 59.85 | 58.46 | 231 | 114 |
100.00%
|
50.00%
|
| Primer 52 | Methanobrevibacter | TGCTGCATGTAGGCGTTGT | TGCAGCTACACCAGTTGGC | 60.30 | 60.60 | 271 | 113 |
100.00%
|
50.00%
|
| Primer 53 | Methanobrevibacter | ACATGAGCGACCCACTTGG | TGGCCATTTGTACTTCAGCCA | 60.00 | 60.20 | 240 | 113 |
100.00%
|
50.00%
|
| Primer 54 | Methanobrevibacter | AGTTGCAGGAATAGCAGCCA | CCAGCTGGGGTCTAGAAATCC | 59.67 | 59.86 | 292 | 113 |
100.00%
|
50.00%
|
| Primer 55 | Methanobrevibacter | GCACTTCCTGTGGCATTGC | GCCTACCCCTGTTGCCATT | 60.08 | 60.00 | 252 | 112 |
100.00%
|
50.00%
|
| Primer 56 | Methanobrevibacter | GCACTTCCTGTGGCATTGC | ACTTGCACTGCCTACCCCT | 60.08 | 60.85 | 261 | 111 |
100.00%
|
50.00%
|
| Primer 57 | Methanobrevibacter | GCACTTCCTGTGGCATTGC | TGCACTGCCTACCCCTGTT | 60.08 | 61.15 | 258 | 111 |
100.00%
|
50.00%
|
| Primer 58 | Methanobrevibacter | AGACAGAGACCCACAACATGT | CACCGCATGCAGCACATTT | 58.95 | 60.08 | 230 | 110 |
100.00%
|
75.00%
|
| Primer 59 | Methanobrevibacter | AGACAGAGACCCACAACATGT | ACCGCATGCAGCACATTT | 58.95 | 58.63 | 229 | 110 |
100.00%
|
75.00%
|
| Primer 60 | Methanobrevibacter | AGACAGAGACCCACAACATGT | ACCGCATGCAGCACATTTT | 58.95 | 59.33 | 229 | 110 |
100.00%
|
75.00%
|