| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 301 | Methanobrevibacter | AGACGTGGTGGAAGAAGGAAA | TTTACCGTCAGCCCACACC | 59.23 | 59.93 | 174 | 67 |
100.00%
|
100.00%
|
| Primer 302 | Methanobrevibacter | ACCCAAGATGCCGTGCAA | ACGACCAGGATGTTGCCT | 59.88 | 58.52 | 188 | 66 |
100.00%
|
50.00%
|
| Primer 303 | Methanobrevibacter | AACCCAAGATGCCGTGCA | ACGACCAGGATGTTGCCT | 59.88 | 58.52 | 189 | 66 |
100.00%
|
50.00%
|
| Primer 304 | Methanobrevibacter | GCAGGAGAAGTGATGGGGTT | TGGCCTTCGCTTTCCTGT | 59.67 | 59.16 | 236 | 66 |
100.00%
|
50.00%
|
| Primer 305 | Methanobrevibacter | GCTGCGTTAAGGGCAAGTG | CAGCAAAAGCAACAGTCCCT | 59.79 | 58.96 | 181 | 66 |
100.00%
|
50.00%
|
| Primer 306 | Methanobrevibacter | TGCGTTAAGGGCAAGTGCT | CAGCAAAAGCAACAGTCCCT | 60.23 | 58.96 | 179 | 66 |
100.00%
|
50.00%
|
| Primer 307 | Methanobrevibacter | TGCTGCGTTAAGGGCAAGT | CAGCAAAAGCAACAGTCCCT | 60.23 | 58.96 | 182 | 66 |
100.00%
|
50.00%
|
| Primer 308 | Methanobrevibacter | TGTTGACATGGGTGCCACA | TTCCGTCACCCCAGTCGTA | 59.77 | 60.23 | 263 | 66 |
100.00%
|
50.00%
|
| Primer 309 | Methanobrevibacter | TGGTTCTTGGTCCTGCTGTT | CTGGTCGGCTGATAAAGTTCA | 59.45 | 58.03 | 244 | 66 |
100.00%
|
50.00%
|
| Primer 310 | Methanobrevibacter | GGTCCAACAGAAGAACCGC | TGCATTCTCCACCAGCACTT | 58.76 | 59.89 | 186 | 65 |
100.00%
|
75.00%
|