| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 311 | Methanobrevibacter | AGGTCCAACAGAAGAACCGC | TGCATTCTCCACCAGCACTT | 60.25 | 59.89 | 187 | 65 |
100.00%
|
75.00%
|
| Primer 312 | Methanobrevibacter | TCCAACAGAAGAACCGCAGA | TGCATTCTCCACCAGCACT | 59.24 | 59.24 | 184 | 65 |
100.00%
|
75.00%
|
| Primer 313 | Methanobrevibacter | AAACTGCACGTGCTATGGC | AGGCCATCCTTCTTGTCCTT | 59.12 | 58.62 | 194 | 65 |
100.00%
|
100.00%
|
| Primer 314 | Methanobrevibacter | TTGCCCACCTCGTTCTGA | AGGAAACAGTTGCAGGACCC | 58.44 | 60.18 | 227 | 65 |
100.00%
|
75.00%
|
| Primer 315 | Methanobrevibacter | ATGGAGACGTCCTAGGGGT | AGCGTCAGTAGCTGGGTTT | 58.98 | 58.94 | 181 | 64 |
100.00%
|
50.00%
|
| Primer 316 | Methanobrevibacter | GCCATCTACAACCACCCCAA | GGCTCCGTCTCCGTGTTTT | 59.96 | 60.30 | 286 | 64 |
100.00%
|
50.00%
|
| Primer 317 | Methanobrevibacter | GCCATCTACAACCACCCCA | AGGCTCCGTCTCCGTGTTT | 59.31 | 61.21 | 287 | 64 |
100.00%
|
50.00%
|
| Primer 318 | Methanobrevibacter | ATGATGAAACCGGACGCAG | AGTTCCGAGGTCTGCTTCTAA | 58.23 | 58.47 | 282 | 63 |
100.00%
|
50.00%
|
| Primer 319 | Methanobrevibacter | TGGAGACGTCCTAGGGGTAA | AGCGTCAGTAGCTGGGTT | 59.00 | 58.21 | 180 | 63 |
100.00%
|
50.00%
|
| Primer 320 | Methanobrevibacter | ACAGTTCCCTGCGGAGTAA | TCCCAGAAACCAGCTGCAG | 58.25 | 59.93 | 176 | 63 |
100.00%
|
50.00%
|