| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1151 | Methanosphaera | GAAGGTGAACGTGTAGGCG | AGTTCACCAGTACCTGCACTG | 58.56 | 59.93 | 257 | 68 |
100.00%
|
66.67%
|
| Primer 1152 | Methanosphaera | TGCTGTTGAAATATCACGCGT | ATAGCTCCAAGGCATGCCG | 58.93 | 60.23 | 168 | 68 |
100.00%
|
66.67%
|
| Primer 1153 | Methanosphaera | TGGCTGCTGTTGCTGGAA | GAGGAGGTGCATATGCCGT | 59.80 | 59.55 | 214 | 68 |
100.00%
|
66.67%
|
| Primer 1154 | Methanosphaera | ATGGCTGCTGTTGCTGGA | GAGGAGGTGCATATGCCGT | 59.56 | 59.55 | 215 | 68 |
100.00%
|
66.67%
|
| Primer 1155 | Methanosphaera | AACAAGTGCTGCCTGTGC | CCCGTAGATATTCCAGCACCA | 58.88 | 59.31 | 180 | 68 |
100.00%
|
66.67%
|
| Primer 1156 | Methanosphaera | GGAATACTCACCTCGGGAGT | AGTGAACCACCATGTGCCA | 58.22 | 59.46 | 200 | 68 |
100.00%
|
66.67%
|
| Primer 1157 | Methanosphaera | ACGCGAATTTGGTGCACA | CACTTTGAGCTCCACCACCA | 58.57 | 60.18 | 224 | 68 |
100.00%
|
66.67%
|
| Primer 1158 | Methanosphaera | ACGCGAATTTGGTGCACA | ACTTTGAGCTCCACCACCA | 58.57 | 58.77 | 223 | 68 |
100.00%
|
66.67%
|
| Primer 1159 | Methanosphaera | AGCCCAGGAAAAGCTGGAC | TTCCTGGTGTGTTTGGTCCT | 59.92 | 59.08 | 262 | 66 |
100.00%
|
33.33%
|
| Primer 1160 | Methanosphaera | GCCCAGGAAAAGCTGGACA | TTCCTGGTGTGTTTGGTCCT | 60.23 | 59.08 | 261 | 66 |
100.00%
|
33.33%
|