| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1161 | Methanosphaera | GCAGGAACAAGCCCAGGAA | TTCCTGGTGTGTTTGGTCCT | 60.23 | 59.08 | 271 | 66 |
100.00%
|
33.33%
|
| Primer 1162 | Methanosphaera | AGCCCAGGAAAAGCTGGA | TTCCTGGTGTGTTTGGTCCT | 58.41 | 59.08 | 262 | 66 |
100.00%
|
33.33%
|
| Primer 1163 | Methanosphaera | TGGAACAGCAGCAGCACTA | TGGTCTGGCCATGTTGCT | 59.25 | 59.15 | 249 | 66 |
100.00%
|
100.00%
|
| Primer 1164 | Methanosphaera | GCAGTTTCACGCGAAGATCG | AGGTCCCCATTTGAAGCAGTT | 60.25 | 59.85 | 264 | 66 |
100.00%
|
33.33%
|
| Primer 1165 | Methanosphaera | ATCACGAGAACACACCCAAGT | TGGGCAGAGCACTTCTCCT | 59.58 | 60.54 | 229 | 66 |
100.00%
|
33.33%
|
| Primer 1166 | Methanosphaera | ATCACGAGAACACACCCAAGT | TGGGCAGAGCACTTCTCCTA | 59.58 | 60.25 | 229 | 66 |
100.00%
|
33.33%
|
| Primer 1167 | Methanosphaera | GGATCAACCGCTGCTATGC | CCCATAACAAGTCCCACACCT | 59.06 | 59.65 | 245 | 66 |
100.00%
|
66.67%
|
| Primer 1168 | Methanosphaera | GGAGGATCAACCGCTGCTA | ACAAGTCCCACACCTGTAGT | 59.18 | 58.20 | 242 | 66 |
100.00%
|
66.67%
|
| Primer 1169 | Methanosphaera | ACGGGAAGAGCATAGACAAGC | CCAATGTCGTGGTTGTGGTT | 60.13 | 58.97 | 295 | 66 |
100.00%
|
66.67%
|
| Primer 1170 | Methanosphaera | CGGGAAGAGCATAGACAAGCA | CCAATGTCGTGGTTGTGGTT | 60.13 | 58.97 | 294 | 66 |
100.00%
|
66.67%
|