| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 121 | Methanosphaera | ATTGCACAAGGAGCACGAG | TTGGTGCTGGGCTTGCTT | 58.45 | 60.12 | 172 | 125 |
100.00%
|
33.33%
|
| Primer 122 | Methanosphaera | ATGATTGCACAAGGAGCACG | TTGGTGCTGGGCTTGCTT | 59.19 | 60.12 | 175 | 125 |
100.00%
|
33.33%
|
| Primer 123 | Methanosphaera | CACAAGGAGCACGAGGAGTA | TTGGTGCTGGGCTTGCTT | 59.11 | 60.12 | 168 | 125 |
100.00%
|
33.33%
|
| Primer 124 | Methanosphaera | GGTGTTCCTGGACCAAGAGG | TGCTTCACAATCCCCTGGTC | 59.96 | 59.96 | 180 | 125 |
100.00%
|
66.67%
|
| Primer 125 | Methanosphaera | GCGTAAGGCATGTTATGAGGA | CCACCACAGGATAGGGCTAGA | 58.17 | 60.41 | 174 | 125 |
100.00%
|
66.67%
|
| Primer 126 | Methanosphaera | AGTCTTGATCCTACGCCATCA | CTGAACCTTTGCCAAAGCCTT | 58.61 | 59.59 | 201 | 125 |
100.00%
|
66.67%
|
| Primer 127 | Methanosphaera | AGTCTTGATCCTACGCCATCA | TGAACCTTTGCCAAAGCCTT | 58.61 | 58.51 | 200 | 125 |
100.00%
|
66.67%
|
| Primer 128 | Methanosphaera | AGGTGGACTGTCAAATGGAGA | TGTTCCACCATATCCTCCCC | 58.66 | 58.48 | 238 | 125 |
100.00%
|
66.67%
|
| Primer 129 | Methanosphaera | TCAGTTGGCGATGTTGGTGT | TGAGCATAAAGAGCAGCACCA | 60.18 | 60.00 | 170 | 124 |
100.00%
|
66.67%
|
| Primer 130 | Methanosphaera | GGATCAACCGCTGCTATGC | TCCCATAACAAGTCCCACACC | 59.06 | 59.65 | 246 | 124 |
100.00%
|
66.67%
|