| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 141 | Methanosphaera | AGTCTTGATCCTACGCCATCA | TCTGAACCTTTGCCAAAGCCT | 58.61 | 60.41 | 202 | 122 |
100.00%
|
100.00%
|
| Primer 142 | Methanosphaera | AGGTGGACTGTCAAATGGAGA | TGTTCCACCATATCCTCCCCT | 58.66 | 59.99 | 238 | 122 |
100.00%
|
100.00%
|
| Primer 143 | Methanosphaera | ACTCCTGCACCACCAGAGT | GAGGACGTCTAGCCCAAAGT | 60.46 | 59.10 | 271 | 121 |
100.00%
|
50.00%
|
| Primer 144 | Methanosphaera | ACTCCTGCACCACCAGAGT | AGGACGTCTAGCCCAAAGTTG | 60.46 | 60.00 | 270 | 121 |
100.00%
|
50.00%
|
| Primer 145 | Methanosphaera | GGTGATGAGGCAAAAGCACG | TGCCCATGCATCACCTTGA | 60.11 | 59.62 | 171 | 121 |
100.00%
|
50.00%
|
| Primer 146 | Methanosphaera | GGCTGTAAAAGGTCATGGAGC | ACATCGTATGTTCCATCGGC | 59.25 | 58.13 | 207 | 121 |
100.00%
|
100.00%
|
| Primer 147 | Methanosphaera | AAGAAGGGGTATGGCCTTGT | TGCACCTAAGACAACCTGTGA | 58.62 | 59.23 | 231 | 121 |
100.00%
|
100.00%
|
| Primer 148 | Methanosphaera | AAAGAAGGGGTATGGCCTTGT | TGCACCTAAGACAACCTGTGA | 59.28 | 59.23 | 232 | 121 |
100.00%
|
100.00%
|
| Primer 149 | Methanosphaera | AGGGGTATGGCCTTGTTTACA | TGCACCTAAGACAACCTGTGA | 59.00 | 59.23 | 227 | 121 |
100.00%
|
100.00%
|
| Primer 150 | Methanosphaera | TCACTGGGCTCTACCTGGA | GTATGTCCTCTCGGGTCACG | 59.22 | 59.62 | 153 | 121 |
100.00%
|
100.00%
|