| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1321 | Methanosphaera | GGCGTGTATGGTGTTTTGGG | TCATCTGCAGCACCATTTGT | 59.76 | 58.09 | 197 | 59 |
100.00%
|
66.67%
|
| Primer 1322 | Methanosphaera | GGCGTGTATGGTGTTTTGGG | ATCTGCAGCACCATTTGTTGT | 59.76 | 59.03 | 195 | 59 |
100.00%
|
66.67%
|
| Primer 1323 | Methanosphaera | GGCGTGTATGGTGTTTTGGG | TGACCACCATGTGTATGTCCA | 59.76 | 59.02 | 296 | 59 |
100.00%
|
66.67%
|
| Primer 1324 | Methanosphaera | AATCAGTCCCACAACAGCAAA | TGGACAACTTCGTTGGGCA | 58.33 | 59.78 | 220 | 59 |
100.00%
|
66.67%
|
| Primer 1325 | Methanosphaera | TTGGAACAGCAGCAGCACT | TGGTCTGGCCATGTTGCTT | 60.15 | 59.85 | 250 | 58 |
100.00%
|
100.00%
|
| Primer 1326 | Methanosphaera | TTGGAACAGCAGCAGCAC | TGGTCTGGCCATGTTGCTT | 58.56 | 59.85 | 250 | 58 |
100.00%
|
100.00%
|
| Primer 1327 | Methanosphaera | AACCCAATGGCTACCGGT | TGAACGAGGTCTGCTCCTT | 58.50 | 58.25 | 227 | 58 |
100.00%
|
33.33%
|
| Primer 1328 | Methanosphaera | AAACCCAATGGCTACCGGT | TGAACGAGGTCTGCTCCTT | 59.23 | 58.25 | 228 | 58 |
100.00%
|
33.33%
|
| Primer 1329 | Methanosphaera | GGAACATGGCGTGCAATGG | AGCAAGTGGTGACCATGCTT | 60.15 | 60.18 | 219 | 58 |
100.00%
|
33.33%
|
| Primer 1330 | Methanosphaera | TGACAATGGCAGTATGGGCTT | ACAGCTCCTGGTGCTAGAAAT | 59.99 | 59.09 | 207 | 58 |
100.00%
|
33.33%
|