| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1331 | Methanosphaera | ACCCTGAACAACTCCGCTT | AGGACATGTTGCTACAGCCAT | 59.16 | 59.72 | 176 | 58 |
100.00%
|
33.33%
|
| Primer 1332 | Methanosphaera | ACAATACGTGAAGCTCCCCTT | AACACAGCTTGCTGTTGGT | 59.37 | 58.11 | 180 | 58 |
100.00%
|
33.33%
|
| Primer 1333 | Methanosphaera | TGTGACTGTGCTGAAGGAGA | TCCCCACGTAACTCACTTGA | 58.59 | 58.29 | 191 | 58 |
100.00%
|
66.67%
|
| Primer 1334 | Methanosphaera | TCAGAGAAAGGGTTGGCAGA | TCCCCACGTAACTCACTTGA | 58.57 | 58.29 | 261 | 58 |
100.00%
|
66.67%
|
| Primer 1335 | Methanosphaera | ACTACTCCTGCACCACCAGA | AGTGGACGTCTTGCCCAT | 60.18 | 58.52 | 275 | 58 |
100.00%
|
66.67%
|
| Primer 1336 | Methanosphaera | CGCCTTCTGAAGCAGGAGA | GCTCCAGCTACAACTCCCA | 59.41 | 59.02 | 298 | 58 |
100.00%
|
66.67%
|
| Primer 1337 | Methanosphaera | CGCCTTCTGAAGCAGGAGA | CCAGCTACAACTCCCACCTG | 59.41 | 60.04 | 295 | 58 |
100.00%
|
66.67%
|
| Primer 1338 | Methanosphaera | TGGCAAATGAAGCTCCTGAGA | GGATCAAGAACAAGGGGTGTG | 59.65 | 58.84 | 151 | 58 |
100.00%
|
66.67%
|
| Primer 1339 | Methanosphaera | TGGAACAGCAGCTGCACTT | TCTTGATCTGGCCATGTTGCT | 60.15 | 59.99 | 252 | 58 |
100.00%
|
66.67%
|
| Primer 1340 | Methanosphaera | AGATGCCCATGTACATGCAGA | TGCCACACGTTTTGGTTCA | 59.51 | 58.43 | 172 | 58 |
100.00%
|
66.67%
|