| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1351 | Methanosphaera | GAAGGTGAACGTGTAGGCG | AGTTCACCAGTACCTGCACT | 58.56 | 58.58 | 257 | 57 |
100.00%
|
66.67%
|
| Primer 1352 | Methanosphaera | GAAGGTGAACGTGTAGGCG | GTTCACCAGTACCTGCACTG | 58.56 | 58.49 | 256 | 57 |
100.00%
|
66.67%
|
| Primer 1353 | Methanosphaera | AAACAAGTGCTGCCTGTGC | CCCGTAGATATTCCAGCACCA | 59.56 | 59.31 | 181 | 57 |
100.00%
|
66.67%
|
| Primer 1354 | Methanosphaera | TGCAGGTGAATGGAGGGGA | CCTCCAGGCCAACGTGTTA | 60.54 | 59.63 | 186 | 56 |
100.00%
|
66.67%
|
| Primer 1355 | Methanosphaera | ACCCCAAGATGTAACAGCAGA | CTCCCTCATCAAGCTCTTCGT | 59.01 | 59.52 | 287 | 56 |
100.00%
|
33.33%
|
| Primer 1356 | Methanosphaera | ACCCCAAGATGTAACAGCAGA | TCCCTCATCAAGCTCTTCGT | 59.01 | 58.44 | 286 | 56 |
100.00%
|
33.33%
|
| Primer 1357 | Methanosphaera | CACCCCAAGATGTAACAGCAG | CTCCCTCATCAAGCTCTTCGT | 58.91 | 59.52 | 288 | 56 |
100.00%
|
33.33%
|
| Primer 1358 | Methanosphaera | CACCCCAAGATGTAACAGCAG | TCCCTCATCAAGCTCTTCGT | 58.91 | 58.44 | 287 | 56 |
100.00%
|
33.33%
|
| Primer 1359 | Methanosphaera | TCAAGTATTCTCAGCACGCGT | CACTCGGTTGTACTAGCAGGT | 60.07 | 59.46 | 183 | 56 |
100.00%
|
33.33%
|
| Primer 1360 | Methanosphaera | TCAAGTATTCTCAGCACGCGT | ACACTCGGTTGTACTAGCAGG | 60.07 | 59.46 | 184 | 56 |
100.00%
|
33.33%
|