| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1451 | Methanosphaera | TGGAACAACCGCAAGTTCAAT | TGCTTCAACTCCAGGACTAGC | 58.97 | 59.72 | 169 | 50 |
100.00%
|
33.33%
|
| Primer 1452 | Methanosphaera | TGACAATGGCAGTATGGGCT | TACAGCTCCTGGTGCTAGAAA | 59.37 | 58.46 | 208 | 50 |
100.00%
|
33.33%
|
| Primer 1453 | Methanosphaera | TGCAGGAGTCGGTGACATG | AGGCCAGAGATCTGCAGCT | 59.71 | 60.69 | 229 | 50 |
100.00%
|
33.33%
|
| Primer 1454 | Methanosphaera | ACCCTGTTAAAGCAGCTGC | GTGTGTTTGAAGGGCTTGCT | 58.35 | 59.25 | 177 | 50 |
100.00%
|
33.33%
|
| Primer 1455 | Methanosphaera | TGTGTTGCGGTGCAGGT | TGCAACGAGTTGTTCTGGGT | 59.76 | 60.11 | 293 | 50 |
100.00%
|
33.33%
|
| Primer 1456 | Methanosphaera | TGATGTGTTGCGGTGCAG | TGCAACGAGTTGTTCTGGGT | 58.66 | 60.11 | 296 | 50 |
100.00%
|
33.33%
|
| Primer 1457 | Methanosphaera | AACAGAGGCAAGGGGAGAA | TTGTCCCAACACCCCTTTGA | 58.13 | 59.37 | 299 | 50 |
100.00%
|
66.67%
|
| Primer 1458 | Methanosphaera | ACTCCTGCACCACCAGAA | AAGTGGACGTCTTGCCCAT | 58.01 | 59.24 | 273 | 50 |
100.00%
|
66.67%
|
| Primer 1459 | Methanosphaera | GCTGTAAAAGGTCATGGAGCT | TCGTATGTTCCATCGGCAACA | 58.28 | 60.07 | 203 | 50 |
100.00%
|
66.67%
|
| Primer 1460 | Methanosphaera | GCTGTAAAAGGTCATGGAGCT | ACATCGTATGTTCCATCGGCA | 58.28 | 59.86 | 206 | 50 |
100.00%
|
66.67%
|