| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1461 | Methanosphaera | TCCTGAGGGGTGGTCAGAA | GCCACATGCTCTTTCTGTGT | 59.45 | 58.76 | 276 | 50 |
100.00%
|
66.67%
|
| Primer 1462 | Methanosphaera | ACTAATCAAAGGCGATGCACT | TGAATGATTGTGGTGGGAGTG | 58.01 | 58.21 | 260 | 49 |
100.00%
|
33.33%
|
| Primer 1463 | Methanosphaera | ACTAATCAAAGGCGATGCACT | GAATGATTGTGGTGGGAGTGT | 58.01 | 58.20 | 259 | 49 |
100.00%
|
33.33%
|
| Primer 1464 | Methanosphaera | ACTGGTGGTGGAAGATTTGCA | ACTTCCAATTACATGCCCTGC | 60.13 | 58.90 | 261 | 49 |
100.00%
|
33.33%
|
| Primer 1465 | Methanosphaera | TCCTGCACTTGGAGCTGT | TGCTCCTTCTTGGCGATCT | 58.43 | 58.71 | 259 | 49 |
100.00%
|
33.33%
|
| Primer 1466 | Methanosphaera | TCCTGCACTTGGAGCTGT | TTGCTCCTTCTTGGCGATCT | 58.43 | 59.38 | 260 | 49 |
100.00%
|
33.33%
|
| Primer 1467 | Methanosphaera | ACACCAAAATACACCCAAGCA | TGCTGTTACTCCCCATATGGA | 58.33 | 58.23 | 153 | 49 |
100.00%
|
33.33%
|
| Primer 1468 | Methanosphaera | ACACCAAAATACACCCAAGCA | GTGCTGTTACTCCCCATATGG | 58.33 | 58.15 | 154 | 49 |
100.00%
|
33.33%
|
| Primer 1469 | Methanosphaera | AGGTGCTGTAGGATCCGGA | GGTGTGCATCTAGGTGGCA | 59.69 | 59.70 | 182 | 49 |
100.00%
|
33.33%
|
| Primer 1470 | Methanosphaera | GCATGACAGTGGCCTAAATGT | TCCTGGTGCTTTAGGTGCA | 58.91 | 58.85 | 295 | 49 |
100.00%
|
66.67%
|