| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1481 | Methanosphaera | GTTGGATGTGGAACTGGGG | GGTTACCTCCACTTCCACCA | 58.04 | 58.94 | 220 | 48 |
100.00%
|
66.67%
|
| Primer 1482 | Methanosphaera | CGGGAAGAGCATAGACAAGC | CGTGGTTGTGGTTTTCTGGG | 58.42 | 59.62 | 287 | 48 |
100.00%
|
66.67%
|
| Primer 1483 | Methanosphaera | AAAGAGGTACGGGAAGAGCA | CGTGGTTGTGGTTTTCTGGG | 58.36 | 59.62 | 296 | 48 |
100.00%
|
66.67%
|
| Primer 1484 | Methanosphaera | GGGGTGGTTACTCTTTGGGG | GTTCCAAGTCCACAACCTGC | 59.96 | 59.33 | 227 | 48 |
100.00%
|
66.67%
|
| Primer 1485 | Methanosphaera | GGGGTGGTTACTCTTTGGGG | TCCAAGTCCACAACCTGCTA | 59.96 | 58.57 | 225 | 48 |
100.00%
|
66.67%
|
| Primer 1486 | Methanosphaera | GAGGGGTGGTTACTCTTTGGG | TTCCAAGTCCACAACCTGCT | 60.00 | 59.45 | 228 | 48 |
100.00%
|
66.67%
|
| Primer 1487 | Methanosphaera | GGTCCAACACCTCTTGCATC | TTGTATTTAGAAGCGCAGGGG | 58.83 | 58.36 | 178 | 48 |
100.00%
|
66.67%
|
| Primer 1488 | Methanosphaera | ACCGAAATTGGTGAAGTCGC | ACCTCAACACCCCTTACAACA | 59.13 | 59.15 | 194 | 47 |
100.00%
|
33.33%
|
| Primer 1489 | Methanosphaera | ACAATGGCGTAAAAGCTCAGT | GTGCTTCTAGTTGGTCTGCTT | 58.50 | 58.23 | 245 | 47 |
100.00%
|
33.33%
|
| Primer 1490 | Methanosphaera | TGGGGACAACGTCAATTCAGA | TTTTCCTGCACGAATGTTGGT | 59.58 | 58.97 | 162 | 47 |
100.00%
|
33.33%
|