| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1491 | Methanosphaera | GGTTACGGAGCTACTAGGCT | TGGTGCCCAGTCTCGTCTT | 58.31 | 60.84 | 172 | 47 |
100.00%
|
33.33%
|
| Primer 1492 | Methanosphaera | AGGTTACGGAGCTACTAGGC | TGGTGCCCAGTCTCGTCTT | 58.31 | 60.84 | 173 | 47 |
100.00%
|
33.33%
|
| Primer 1493 | Methanosphaera | TGGACATTCCATACACACGC | CCCCATGCCCATACAGCAA | 58.27 | 60.08 | 172 | 47 |
100.00%
|
66.67%
|
| Primer 1494 | Methanosphaera | AGGCACTTGGGGAAATGGA | TCACCAGTTGCTGCCCTT | 58.83 | 59.07 | 166 | 47 |
100.00%
|
66.67%
|
| Primer 1495 | Methanosphaera | TGCACAGCAGGAACCTCA | AGCAATTGCAGGCCAAATAGA | 58.76 | 58.54 | 253 | 47 |
100.00%
|
66.67%
|
| Primer 1496 | Methanosphaera | AGCAGACAAAGCCTCAGAAGA | ACGTTTTGGTGGATGGGGAA | 59.30 | 59.81 | 211 | 47 |
100.00%
|
66.67%
|
| Primer 1497 | Methanosphaera | GATGCTGAACTTGCTGCAGA | TGGTGGATGGGGAAGTTTTGT | 58.84 | 59.78 | 297 | 47 |
100.00%
|
66.67%
|
| Primer 1498 | Methanosphaera | AGATGCTGAACTTGCTGCAGA | TGGTGGATGGGGAAGTTTTGT | 60.27 | 59.78 | 298 | 47 |
100.00%
|
66.67%
|
| Primer 1499 | Methanosphaera | AGGAGGACCAAAACTAGCTCG | TCGCGTCTTGCTCCTATCA | 59.45 | 58.51 | 159 | 46 |
100.00%
|
33.33%
|
| Primer 1500 | Methanosphaera | GGTGGAGCAAATGCAGTGC | GCAAGAAGTGGAAGTCCCCA | 60.08 | 59.89 | 281 | 46 |
100.00%
|
66.67%
|